Molecular assessment of Bartonella in Gerbillus nanus from Saudi Arabia reveals high levels of prevalence, diversity and co-infection

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dc.contributor.author Kleynhans, D.J. (Dewald)
dc.contributor.author Sarlia, Joshua
dc.contributor.author Hatyoka, L.M. (Luiza)
dc.contributor.author Alagaili, Abdulaziz N.
dc.contributor.author Bennett, Nigel C., 1961-
dc.contributor.author Mohammed, Osama B.
dc.contributor.author Bastos, Armanda D.S.
dc.date.accessioned 2019-04-17T12:10:38Z
dc.date.issued 2018-11
dc.description.abstract Bartonellae bacteria are associated with several re-emerging human diseases. These vector-borne pathogens have a global distribution, yet data on Bartonella prevalence and diversity in the Arabian Peninsula are limited. In this study we assessed the Bartonella infection status of the Baluchistan gerbil (Gerbillus nanus), a species associated with pastoral communities throughout the Middle East region, using a multi-gene PCR screening approach. The results demonstrated that 94 (68.1%) of the 138 gerbils trapped on a monthly basis, over a period of one year, were PCR-positive. Sequencing of the gltA gene region confirmed the presence of four discrete Bartonella lineages (I–IV) and high levels of co-infection (33.0%). Each of the four lineages, varied in overall abundance (7.5%–47.9%) and had discernible seasonal peaks. Bartonella status was significantly correlated with ectoparasite presence, but not with sex, nor with season. Statistical analyses further revealed that co-infected individuals had a significantly higher relative body condition. Multi-locus sequence analysis (MLSA) performed with a concatenated dataset of three genetic loci (gltA, nuoG, and rpoB), 1452 nucleotides (nt) in length confirmed that lineage IV, which occurred in 24 PCR-positive animals (25.5%), is most closely related to zoonotic B. elizabethae. The remaining three lineages (I-III) formed a monophyletic clade which, on the basis of gltA was shown to contain bartonellae from diverse Gerbillinae species from the Middle East, suggestive of a gerbil-associated species complex in this region. Lineage I was identical to a Candidatus B. sanaae strain identified previously in Bushy-tailed jirds (Sekeetamys calurus) from Egypt, wherease MLSA indicate that lineages II and III are novel. The high levels of infection and co-infection, together with the presence of multiple Bartonella lineages indicate that Gerbillus nanus is likely a natural reservoir of Bartonella in the Arabian Peninsula. en_ZA
dc.description.department Mammal Research Institute en_ZA
dc.description.department Zoology and Entomology en_ZA
dc.description.embargo 2019-11-01
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship The National Research Foundation (NRF) of South Africa through individual (ADSB, No: 201893), SARCHI Chair of Mammal Behavioural Ecology and Physiology (NCB, No: 64756) and facilities (No: UID78566) grants, as well as the Vice Deanship of Research Chairs at the King Saud University. en_ZA
dc.description.uri http://www.elsevier.com/locate/meegid en_ZA
dc.identifier.citation Kleynhans, D.J., Sarlia, J., Hatyoka, L.M., et al. 2018, 'Molecular assessment of Bartonella in Gerbillus nanus from Saudi Arabia reveals high levels of prevalence, diversity and co-infection', Infection, Genetics and Evolution, vol. 65, pp. 244-250. en_ZA
dc.identifier.issn 1567-1348 (print)
dc.identifier.issn 1567-7257 (online)
dc.identifier.other 10.1016/j.meegid.2018.07.036
dc.identifier.uri http://hdl.handle.net/2263/69004
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Infection, Genetics and Evolution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Infection, Genetics and Evolution, vol. 65, pp. 244-250, 2018, doi : 10.1016/j.meegid.2018.07.036. en_ZA
dc.subject Multi-locus sequence analysis (MLSA) en_ZA
dc.subject Seasonal variation en_ZA
dc.subject Middle East en_ZA
dc.subject Gerbillinae en_ZA
dc.subject Bartonella elizabethae en_ZA
dc.subject Candidatus B. sanaae en_ZA
dc.title Molecular assessment of Bartonella in Gerbillus nanus from Saudi Arabia reveals high levels of prevalence, diversity and co-infection en_ZA
dc.type Postprint Article en_ZA


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