Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bull semen

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dc.contributor.author Annandale, C.H. (Cornelius Henry)
dc.contributor.author Smuts, M.P. (Mario)
dc.contributor.author Ebersohn, Karen
dc.contributor.author Du Plessis, Lizette
dc.contributor.author Venter, Estelle Hildegard
dc.contributor.author Stout, T.A.E. (Tom)
dc.date.accessioned 2019-04-03T10:35:06Z
dc.date.issued 2018-08
dc.description.abstract Lumpy skin disease is an economically important disease of cattle, caused by the lumpy skin disease virus (LSDV; Capripoxvirus). It has a variable clinical appearance but, in severely affected animals, is associated with extensive skin damage, pneumonia and death. The LSDV can be found in the semen of infected bulls for prolonged periods of time, from where it can be transmitted by mating or artificial insemination and cause clinical disease in heifers and cows. In this study, an ejaculate was collected from a LSDV seronegative bull and confirmed free from LSDV DNA by PCR. The ejaculate was split into a control sample (C), a sample spiked with a 4 log TCID50 dose of an LSDV isolate (HD) and a 103 dilution of the virus suspension (ND) and frozen routinely. Two straws from each of the different semen treatment groups (HD, ND and C) were subsequently thawed and subjected to swim-up, single layer centrifugation, Percoll® density gradient and a Percoll® density gradient with added trypsin. For one set of straws, semen quality variables were recorded, and viral DNA status determined using PCR; the other set was used for positive staining electron microscopy. Samples determined to be positive for LSDV DNA by PCR were then subjected to virus isolation (VI). Complete elimination of LSDV from semen did not occur with use of any of the processing methods. Trypsin did reduce the viral load, and eliminated LSDV from the ND sample, but severely negatively influenced semen quality. The LSDV virions, as assessed by electron microscopy, were associated with the sperm plasma membrane. Further investigation is needed to establish the efficacy of immuno-extenders for rendering semen free from LSDV. en_ZA
dc.description.department Anatomy and Physiology en_ZA
dc.description.department Production Animal Studies en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.embargo 2019-08-01
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship A grant from the Health and Welfare Sector Education and Training Authority (HWSETA), Department: Higher Education and Training, South Africa. en_ZA
dc.description.uri http://www.elsevier.com/locate/anireprosci en_ZA
dc.identifier.citation Annandale, C.H., Smuts, M.P., Ebersohn, K. et al. 2018, 'Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bull semen', Animal Reproduction Science, vol. 195, pp. 24-29. en_ZA
dc.identifier.issn 0378-4320 (print)
dc.identifier.issn 1873-2232 (online)
dc.identifier.other 10.1016/j.anireprosci.2018.04.080
dc.identifier.uri http://hdl.handle.net/2263/68762
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Animal Reproduction Science. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Animal Reproduction Science, vol. 195, pp. 24-29. 2018. doi : 10.1016/j.anireprosci.2018.04.080. en_ZA
dc.subject Lumpy skin disease virus (LSDV) en_ZA
dc.subject Bull en_ZA
dc.subject Semen en_ZA
dc.subject Sanitation methods en_ZA
dc.title Effect of semen processing methods on lumpy skin disease virus status in cryopreserved bull semen en_ZA
dc.type Postprint Article en_ZA


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