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dc.contributor.author | Rademan, Rochelle![]() |
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dc.contributor.author | Markotter, Wanda![]() |
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dc.contributor.author | Paweska, Janusz Tadeusz![]() |
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dc.contributor.author | Jansen van Vuren, Petrus![]() |
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dc.date.accessioned | 2019-03-07T10:13:21Z | |
dc.date.issued | 2019-04 | |
dc.description.abstract | Zika (ZIKV) and Spondweni viruses (SPOV) are closely related mosquito borne flaviviruses in the Spondweni serogroup. The co-circulation and similar disease presentation following ZIKV and SPOV infection necessitates the development of a diagnostic tool for their simultaneous detection and distinction. We developed a one-step multiplex real-time RT-PCR (ZIKSPOV) to detect and distinguish between SPOV and ZIKV by utilizing a single primer set combined with virus specific hydrolysis probes. The ZIKSPOV assay was compared to published virus specific real-time RT-PCR assays and the limit of detection was comparable. The SPOV reference strain AR94 was detectable to 0.001 TCID50 per PCR reaction, while African lineage ZIKV (MR 766) was detectable to 0.002 TCID50 per reaction and Asian lineage ZIKV (H/PF/2013) to 0.05 TCID50 per reaction. The ZIKSPOV assay did not detect other flaviviruses, indicative of its specificity for Spondweni serogroup. The ZIKSPOV assay is a useful addition to arbovirus diagnostic and surveillance tools in areas where ZIKV and SPOV are expected to co-circulate. Further evaluation is required to demonstrate the application of the assay for detection of ZIKV and SPOV in mosquito and human clinical samples. | en_ZA |
dc.description.department | Medical Virology | en_ZA |
dc.description.embargo | 2020-04-01 | |
dc.description.librarian | hj2019 | en_ZA |
dc.description.sponsorship | The Centre for Emerging Zoonotic and Parasitic Diseases, National Institute of Communicable Diseases (NICD), a division of the National Health Laboratory Services. Ms. Rochelle Rademan was supported by a grant-linked National Research Foundation bursary supplied by the NRF-DST South African Research Chair in Infectious Diseases of Animals (Zoonoses) (grant no. 98339). Further financial support was obtained from the Poliomyelitis Research Foundation (grant no. 18/40). | en_ZA |
dc.description.uri | http://www.elsevier.com/locate/jviromet | en_ZA |
dc.identifier.citation | Rademan, R., Markotter, W., Paweska, J.T. et al. 2019, 'Multiplex real-time RT-PCR for detection and distinction of Spondweni and Zika virus', Journal of Virological Methods, vol. 266, pp. 72-76. | en_ZA |
dc.identifier.issn | 0166-0934 (print) | |
dc.identifier.issn | 1879-0984 (online) | |
dc.identifier.other | 10.1016/j.jviromet.2019.01.011 | |
dc.identifier.uri | http://hdl.handle.net/2263/68606 | |
dc.language.iso | en | en_ZA |
dc.publisher | Elsevier | en_ZA |
dc.rights | © 2019 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Virological Methods. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Journal of Virological Methods, vol. 266, pp. 72-76, 2019. doi : 10.1016/j.jviromet.2019.01.011. | en_ZA |
dc.subject | Aedes | en_ZA |
dc.subject | Arbovirus | en_ZA |
dc.subject | Zika virus | en_ZA |
dc.subject | Spondweni virus (SPOV) | en_ZA |
dc.subject | Multiplex | en_ZA |
dc.subject | Flavivirus | en_ZA |
dc.subject | Flaviviridae | en_ZA |
dc.title | Multiplex real-time RT-PCR for detection and distinction of Spondweni and Zika virus | en_ZA |
dc.type | Postprint Article | en_ZA |