Anti-inflammatory and antioxidant properties of leaf extracts of eleven South African medicinal plants used traditionally to treat inflammation

Show simple item record Ondua, Moise Njoya, Emmanuel Mfotie Abdalla, Muna Ali McGaw, Lyndy Joy 2019-02-06T10:01:34Z 2019-04
dc.description.abstract ETHNOPHARMACOLOGICAL RELEVANCE : Inflammation is a complex mechanism employed by the body to promote healing and restoration to normal function in the event of injury. Eleven plant species were selected in this study based on their use in traditional medicine against inflammation in South Africa. METHODS : Hexane, acetone, ethanol, methanol and water extracts of the powdered plants were prepared and a total of fifty-five extracts were tested for their anti-inflammatory and antioxidant activities. The anti-inflammatory activity of extracts was evaluated via the 15-lipoxygenase (15-LOX) inhibitory and the nitric oxide (NO) inhibition assays using lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophages. Total flavonoid and total phenolic contents were determined. The antioxidant activity of the extracts was performed using radical scavenging DPPH (2, 2-diphenyl-1-picrylhydrazyl) and electron reducing ABTS (2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assays. RESULTS : The hexane extract of Typha capensis (TC) had good lipoxygenase inhibitory activity with IC50 of 4.65 µg/mL, significantly (p < 0.05) higher than that of the positive control quercetin (IC50 = 24.60). The same extract also had good nitric oxide inhibitory activity with 86% NO inhibition and cell viability of 97% at 50 µg/mL. The TC acetone extract had the best antioxidant activity with IC50 of 7.11 and 1.91 µg/mL respectively in the DPPH and ABTS assays. Following fractionation of the TC plant material, the ethyl acetate fraction had interesting antioxidant activity and the methanol/water (35%) and hexane fractions had good 15-LOX inhibitory activity. The antioxidant and anti-inflammatory activities therefore resided in both polar and more non-polar fractions. CONCLUSION : The acetone extract of Typha capensis and its fractions had good anti-inflammatory and antioxidant activities, supporting the medicinal use of this species against inflammation. Other species including Ficus elastica, Carpobrotus edulis, Cotyledon orbiculata and Senna italica also had good activity worthy of further investigation. en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.embargo 2020-04-24
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship The National Research Foundation , South Africa (grant number 105993 ) is thanked for providing research funding. The NRF and University of Pretoria , South Africa are acknowledged for doctoral fellowships to OM. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Ondua, M., Njoya, E.M., Abdalla, M.A. et al. 2019, 'Anti-inflammatory and antioxidant properties of leaf extracts of eleven South African medicinal plants used traditionally to treat inflammation', Journal of Ethnopharmacology, vol. 234, pp. 27-35. en_ZA
dc.identifier.issn 0378-8741 (print)
dc.identifier.issn 1872-7573 (online)
dc.identifier.other 10.1016/j.jep.2018.12.030
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Elsevier B.V. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Journal of Ethnopharmacology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Journal of Ethnopharmacology, vol. 234, pp. 27-35. 2019. doi : 10.1016/j.jep.2018.12.030. en_ZA
dc.subject 15-lipoxygenase (15-LOX) en_ZA
dc.subject Anti-inflammatory en_ZA
dc.subject Antioxidant en_ZA
dc.subject Total flavonoid content en_ZA
dc.subject Total phenolic content en_ZA
dc.subject Typha capensis (TC) en_ZA
dc.subject Lipopolysaccharide (LPS) en_ZA
dc.subject Nitric oxide (NO) en_ZA
dc.subject Inflammation en_ZA
dc.subject South African medicinal plants en_ZA
dc.subject Leaf extracts en_ZA
dc.title Anti-inflammatory and antioxidant properties of leaf extracts of eleven South African medicinal plants used traditionally to treat inflammation en_ZA
dc.type Postprint Article en_ZA

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