This review considers current knowledge surrounding species boundaries of the Armillaria
root-rot pathogens and their distribution. In addition, a phylogenetic tree using translation elongation
factor subunit 1-alpha (tef -1 ) from isolates across the globe are used to present a global phylogenetic
framework for the genus. Defining species boundaries based on DNA sequence-inferred phylogenies
has been a central focus of contemporary mycology. The results of such studies have in many cases
resolved the biogeographic history of species, mechanisms involved in dispersal, the taxonomy of
species and how certain phenotypic characteristics have evolved throughout lineage diversification.
Such advances have also occurred in the case of Armillaria spp. that include important causal agents
of tree root rots. This commenced with the first phylogeny for Armillaria that was based on IGS-1
(intergenic spacer region one) DNA sequence data, published in 1992. Since then phylogenies were
produced using alternative loci, either as single gene phylogenies or based on concatenated data.
Collectively these phylogenies revealed species clusters in Armillaria linked to their geographic
distributions and importantly species complexes that warrant further research.
Table S1: Summary of genes and genomic regions employed in phylogenetic studies of Armillaria species.
Table S2: Curated database of published DNA sequences from GenBank for Armillaria species.
Table S3: Additional tef -1 DNA sequences from Armillaria species or taxa included in this study.
Table S4: Distribution of Armillaria species and taxa from phylogenetic studies and their associated biological species designation.
Figure S1. Phylogenetic
trees generated from publications for species for which tef -1 DNA are not available or that had conflicting
phylogenetic positions based on genomic regions other than tef -1 .