Cross reactive immune responses in cattle arising from exposure to Mycobacterium bovis and non-tuberculous mycobacteria

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dc.contributor.author Jenkins, Akinbowale Olajide
dc.contributor.author Gormley, Eamonn
dc.contributor.author Gcebe, Nomakorinte
dc.contributor.author Fosgate, Geoffrey T.
dc.contributor.author Conan, Anne
dc.contributor.author Aagaard, Claus Sindbjerg
dc.contributor.author Michel, Anita Luise
dc.contributor.author Rutten, Victor P.M.G.
dc.date.accessioned 2019-01-21T11:31:19Z
dc.date.issued 2018-04
dc.description.abstract Accurate diagnosis of tuberculosis in cattle may be compromised in areas where there are high rates of exposure to environmental/non-tuberculous mycobacteria (NTM). This cross reaction of immune responses to Mycobacterium bovis antigens shared with NTMs can result in reduced specificity of commonly used diagnostic tests including tuberculin skin tests and the interferon gamma assay (IFN-ɣ). In this study we assessed the cross-reactive immune responses of M. bovis (infected) and NTM exposed animals to M. bovis and M. avium tuberculin, the ESAT6/CFP10 cocktail antigen, tuberculin derived from cultures of selected NTMs, and a panel of recombinant mycobacterium tuberculosis complex (MTBC) antigens sharing homology with orthologues in NTM. Gamma interferon (IFN-ɣ) responses were measured in whole blood cultures using the IFN-ɣ assay and the IFN-ɣ elispot assay on purified peripheral blood mononuclear cells (PBMC). We observed the expected strong IFN-ɣ response to PPD-B in the M. bovis infected animals that distinguished this group from non-infected NTM exposed cattle. The IFN-ɣ responses to PPD-N (M. nonchromogenicum), were relatively high in both infected and non-infected NTM exposed cattle, but were not significantly different to classify the true infection status of each group. The results indicated that the cross-reactive responses to PPD-B and/or PPD-A with PPD-N, likely arose from prior exposure to environmental non-tuberculous mycobacteria. The IFN-ɣ immune responses to the 10 R-Mag measured by the IFN-ɣ elispot assay revealed that three of the selected antigens, Rv3615 (ESpC), Rv0287 (esxG) and the ESAT6/CFP10, were immunogenic in the infected cattle, and distinguished the infected cattle from the non-infected NTM exposed animals. The combined data of PPDs and R-Mags derived from NTM mycobacteria may prove useful in future development of novel bTB diagnostic tests. en_ZA
dc.description.department Production Animal Studies en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.embargo 2019-04-01
dc.description.librarian hj2019 en_ZA
dc.description.uri http://www.elsevier.com/locate/prevetmed en_ZA
dc.identifier.citation Jenkins, A.O., Gormley, E., Gcebe, N. et al. 2018, 'Cross reactive immune responses in cattle arising from exposure to Mycobacterium bovis and non-tuberculous mycobacteria', Preventive Veterinary Medicine, vol. 152, pp. 16-22. en_ZA
dc.identifier.issn 0167-5877 (print)
dc.identifier.issn 1873-1716 (online)
dc.identifier.other 10.1016/j.prevetmed.2018.02.003
dc.identifier.uri http://hdl.handle.net/2263/68193
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Published by Elsevier B.V. Notice : this is the author’s version of a work that was accepted for publication in Preventive Veterinary Medicine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Preventive Veterinary Medicine, vol. 152, pp. 16-22, 2018. doi : 10.1016/j.prevetmed.2018.02.003. en_ZA
dc.subject Non-tuberculous mycobacteria (NTM) en_ZA
dc.subject Interferon gamma assay (IFN-ɣ) en_ZA
dc.subject Mycobacterium tuberculosis complex (MTBC) en_ZA
dc.subject Purified peripheral blood mononuclear cells (PBMC) en_ZA
dc.subject Skin test en_ZA
dc.subject Tuberculosis (TB) en_ZA
dc.subject Diagnosis en_ZA
dc.subject Antigens en_ZA
dc.subject ESAT6 en_ZA
dc.subject Vaccination en_ZA
dc.subject Peptides en_ZA
dc.subject CFP 10 en_ZA
dc.subject Assay en_ZA
dc.subject BCG en_ZA
dc.title Cross reactive immune responses in cattle arising from exposure to Mycobacterium bovis and non-tuberculous mycobacteria en_ZA
dc.type Postprint Article en_ZA


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