Antigen-specific CD4+ and CD8+ T-cell responses in PBMC from pony mares immunized with either native or recombinant zona pellucida vaccines

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dc.contributor.author Joone, C.J. (Carolynne)
dc.contributor.author Schulman, M.L. (Martin)
dc.contributor.author Fosgate, Geoffrey T.
dc.contributor.author Plagis, T.A.
dc.contributor.author Crafford, Jan Ernst
dc.contributor.author Gupta, Satish K.
dc.contributor.author Bertschinger, H.J. (Hendrik Jan)
dc.date.accessioned 2019-01-16T05:22:04Z
dc.date.issued 2019-03
dc.description.abstract Few studies have investigated the cell mediated immune response during zona pellucida-based immunocontraception, despite hypothesized cytotoxic T-cell involvement in ovarian dysfunction associated with these vaccines. This study aimed to investigate antigen-specific anamnestic responses of helper (CD4+) and cytotoxic (CD8+) T-lymphocytes in peripheral blood mononuclear cells (PBMC) isolated from pony mares before and after their treatment with native porcine zona pellucida (pZP), recombinant pZP3 and pZP4 antigens (reZP) or adjuvanted saline. Mares were randomly assigned to pZP, reZP and control groups (n = 7 per group). Treatments consisted of a primary vaccination or saline (V1; Day 0) incorporating Freund's modified complete adjuvant, followed by a single booster (V2; Day 35) incorporating Freund's incomplete adjuvant. Peripheral blood mononuclear cells were isolated and cryopreserved immediately prior to V1 (Day 0) and five weeks post V2 (Day 70). Relative proliferation of T-lymphocytes in response to pZP antigen was assessed using carboxyfluorescein diacetate succinimidyl ester dilution with immunophenotyping, analysed via flow cytometry. Significant pZP-specific CD4+ and CD8+ T-lymphocyte responses were detected in PBMC isolated from mares treated with either pZP or reZP, in comparison to pre-treatment samples. In the pZP group, but not the reZP group, CD8+ T-cell proliferation showed significant negative correlations to circulating progesterone, oestradiol and anti-Müllerian hormone levels. Results suggest that antigen-specific CD8+ T-cells may play a role in ovarian suppression observed during pZP immunocontraception in this species. en_ZA
dc.description.department Production Animal Studies en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.embargo 2020-03-01
dc.description.librarian hj2019 en_ZA
dc.description.sponsorship The Technology Innovation Agency, South Africa (TAHC12 - 00042). en_ZA
dc.description.uri http://www.theriojournal.com en_ZA
dc.identifier.citation Joonè, C.J., Schulman, M.L., Fosgate, G.T. et al. 2019, 'Antigen-specific CD4+ and CD8+ T-cell responses in PBMC from pony mares immunized with either native or recombinant zona pellucida vaccines', Theriogenology, vol. 126, pp. 106-113. en_ZA
dc.identifier.issn 0093-691X (print)
dc.identifier.issn 1879-3231 (online)
dc.identifier.other 10.1016/j.theriogenology.2018.12.012
dc.identifier.uri http://hdl.handle.net/2263/68154
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Theriogenology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Theriogenology, vol. 126, pp. 106-113, 2019. doi : 10.1016/j.theriogenology.2018.12.012. en_ZA
dc.subject Cell mediated immune response en_ZA
dc.subject Cytotoxic T-cells en_ZA
dc.subject Horse (Equus caballus) en_ZA
dc.subject Immunocontraception en_ZA
dc.subject T-lymphocytes en_ZA
dc.subject Peripheral blood mononuclear cell (PBMC) en_ZA
dc.subject Porcine zona pellucida (pZP) en_ZA
dc.title Antigen-specific CD4+ and CD8+ T-cell responses in PBMC from pony mares immunized with either native or recombinant zona pellucida vaccines en_ZA
dc.type Postprint Article en_ZA


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