dc.contributor.author |
Laubscher, M.
|
|
dc.contributor.author |
Brown, Katrien
|
|
dc.contributor.author |
Tonfack, L.B.
|
|
dc.contributor.author |
Myburg, Alexander Andrew
|
|
dc.contributor.author |
Mizrachi, Eshchar
|
|
dc.contributor.author |
Hussey, Steven Grant
|
|
dc.date.accessioned |
2018-11-16T08:11:36Z |
|
dc.date.available |
2018-11-16T08:11:36Z |
|
dc.date.issued |
2018-07-20 |
|
dc.description |
M.L. prepared the constructs, performed the protoplast RNA-seq analyses and yeast transactivation experiments,
and co-wrote the manuscript with S.H. K.B., L.T. and S.H. conducted promoter::GUS analyses and drafted these
sections of the manuscript. E.M. and A.M. edited the manuscript, co-supervised the study with S.H. and assisted
with funding. |
en_ZA |
dc.description.abstract |
Secondary cell wall (SCW) deposition in Arabidopsis is regulated among others by NAC transcription
factors, where SND1 chiefly initiates xylem fibre differentiation while VND6 controls metaxylem vessel
SCW development, especially programmed cell death and wall patterning. The translational relevance
of Arabidopsis SCW regulation theory and the utility of characterized transcription factors as modular
synthetic biology tools for improving commercial fibre crops is unclear. We investigated inter-lineage
gene activation dynamics for potential fibre and vessel differentiation regulators from the widely
grown hardwood Eucalyptus grandis (Myrtales). EgrNAC26, a VND6 homolog, and EgrNAC61, an SND1
homolog, were transiently expressed in Arabidopsis mesophyll protoplasts in parallel to determine
early and late (i.e. 7 and 14 hours post-transfection) gene targets. Surprisingly, across the time series
EgrNAC26 activated only a subset of SCW-related transcription factors and biosynthetic genes
activated by EgrNAC61, specializing instead in targeting vessel-specific wall pit and programmed cell
death markers. Promoters of EgrNAC26 and EgrNAC61 both induced reporter gene expression in vessels
of young Arabidopsis plants, with EgrNAC61 also conferring xylem- and cork cambium-preferential
expression in Populus. Our results demonstrate partial conservation, with notable exceptions, of SND1
and VND6 homologs in Eucalyptus and a first report of cork cambium expression for EgrNAC61. |
en_ZA |
dc.description.department |
Biochemistry |
en_ZA |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_ZA |
dc.description.department |
Genetics |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
am2018 |
en_ZA |
dc.description.sponsorship |
A strategic (Eucalyptus Genomics Platform) grant from the Department of Science and Technology (DST) and the National Research Foundation of South Africa (NRF Bioinformatics and Functional Genomics Programme grant UID 97911). Seed funding was provided by the Genomics Research Institute (University of Pretoria). Finally, the work was supported by Sappi and Mondi through the Forest Molecular Genetics (FMG) Programme and matching funding from the Technology and Human Resources for Industry Programme (THRIP gr ant UID 96413). |
en_ZA |
dc.description.uri |
http://www.nature.com/srep |
en_ZA |
dc.identifier.citation |
Laubscher, M.; Brown, K.; Myburg, A. A. et al. 2018, 'Temporal analysis of Arabidopsis genes activated by Eucalyptus grandis NAC transcription factors associated with xylem fibre and vessel development',Scientific Reports, vol. 8, art. 10983, pp. 1-14. |
en_ZA |
dc.identifier.issn |
2045-2322 (online) |
|
dc.identifier.other |
10.1038/s41598-018-29278-w |
|
dc.identifier.uri |
http://hdl.handle.net/2263/67276 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Nature Publishing Group |
en_ZA |
dc.rights |
© The Author(s) 2018. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. |
en_ZA |
dc.subject |
VND6 |
en_ZA |
dc.subject |
Arabidopsis |
en_ZA |
dc.subject |
EgrNAC61 |
en_ZA |
dc.subject |
Secondary cell wall (SCW) |
en_ZA |
dc.subject |
Master switches |
en_ZA |
dc.subject |
Functional analysis |
en_ZA |
dc.subject |
Lignin biosynthesis |
en_ZA |
dc.subject |
Secondary wall biosynthesis |
en_ZA |
dc.subject |
Programmed cell death (PCD) |
en_ZA |
dc.subject |
Tracheary element differentiation |
en_ZA |
dc.subject |
Vascular-related NAC-Domain7 |
en_ZA |
dc.subject |
NAC domain protein family |
en_ZA |
dc.subject |
Wood formation |
en_ZA |
dc.subject |
Populus trichocarpa |
en_ZA |
dc.title |
Temporal analysis of Arabidopsis genes activated by Eucalyptus grandis NAC transcription factors associated with xylem fibre and vessel development |
en_ZA |
dc.type |
Article |
en_ZA |