Characterization of Staphylococcus aureus isolated from milk samples of dairy cows in small holder farms of North-Western Ethiopia

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dc.contributor.author Mekonnen, S.A.
dc.contributor.author Lam, T.J.G.M.
dc.contributor.author Hoekstra, J.
dc.contributor.author Rutten, Victor P.M.G.
dc.contributor.author Tessema, T.S.
dc.contributor.author Broens, E.M.
dc.contributor.author Riesebos, A.E.
dc.contributor.author Spaninks, M.P.
dc.contributor.author Koop, G.
dc.date.accessioned 2018-10-18T06:23:41Z
dc.date.available 2018-10-18T06:23:41Z
dc.date.issued 2018-08-23
dc.description Additional file 1: Dataset supporting the conclusions of this article. en_ZA
dc.description Additional file 2: Percentage resistance to antimicrobials by spa-type for 79 S. aureus isolates. en_ZA
dc.description.abstract BACKGROUND : Staphylococcus aureus is a contagious, opportunistic pathogen that causes clinical or subclinical mastitis in dairy cattle. The genetic background and antimicrobial resistance of isolates from Ethiopian dairy farms has not been studied. Therefore, the aim of this study was to characterize S. aureus from Ethiopian hand milked dairy cows, by spa, MLST and virulence factor typing, and by assessment of antimicrobial susceptibility. A total of 79 S. aureus isolates from intramammary infections was studied. A PCR was used to detect lukM-lukF’ and pvl genes encoding the bovine and human associated bi-component leukocidins, and the toxic shock syndrome toxin gene-1 (tst). Antimicrobial susceptibility was determined using the broth microdilution method. RESULTS : Twenty different spa types were identified, most isolates were t042 (58%), and the closely related t15786 (11%). The proportion of isolates positive for lukM-lukF’, tst and pvl was low at 0.04, 0.10 and 0.09 respectively, with lukM-lukF’ often co-occurring with tst, but notwith pvl. Methicillin-resistance was not found, but resistance to penicillin/ ampicillin (86%) and tetracycline (54%) was very common. CONCLUSIONS : We found a high degree of relatedness among bovine S. aureus isolates in North-Western Ethiopia, suggesting contagious within and between farm transmission of strains that are often resistant to commonly used antimicrobials. This highlights the need for effective preventive measures that aim at limiting transmission of bacteria rather than using antimicrobials to control S. aureus mastitis in Ethiopia. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship The Netherlands Organization for International Cooperation in Higher Education (Nuffic) and the Gustav Rosenberger Memorial Fund. en_ZA
dc.description.uri https://bmcvetres.biomedcentral.com en_ZA
dc.identifier.citation Mekonnen, S.A., Lam, T.J.G.M., Hoekstra, J. et al. 2018, 'Characterization of Staphylococcus aureus isolated from milk samples of dairy cows in small holder farms of North-Western Ethiopia', BMC Veterinary Research, vol. 14, art. 246, pp. 1-8. en_ZA
dc.identifier.issn 1746-6148 (online)
dc.identifier.other 10.1186/s12917-018-1558-1
dc.identifier.uri http://hdl.handle.net/2263/66938
dc.language.iso en en_ZA
dc.publisher BioMed Central en_ZA
dc.rights © The Author(s). 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). en_ZA
dc.subject Staphylococcus aureus en_ZA
dc.subject Spa en_ZA
dc.subject Virulence factor en_ZA
dc.subject Antimicrobial resistance en_ZA
dc.subject Mastitis en_ZA
dc.subject Ethiopia en_ZA
dc.subject Multilocus sequence typing (MLST) en_ZA
dc.subject Strains en_ZA
dc.subject Virulence genes en_ZA
dc.subject Subclinical mastitis en_ZA
dc.subject Intramammary infections en_ZA
dc.subject Molecular characterization en_ZA
dc.subject Antimicrobial susceptibility en_ZA
dc.subject Methicillin-resistant en_ZA
dc.subject Bovine Staphylococcus en_ZA
dc.subject Enterotoxin gene profile en_ZA
dc.title Characterization of Staphylococcus aureus isolated from milk samples of dairy cows in small holder farms of North-Western Ethiopia en_ZA
dc.type Article en_ZA


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