Figure S1. (1) Colony morphology of (a) Bacillus endophyticus that is small circular, wet and non-mucoid and (b) B. anthracis appear circular, mucoid on nutrient agar supplemented with sodium bicarbonate at 5% CO2 after incubation at 37 °C. Colony morphology of B. endophyticus and B. anthracis on sheep blood agar incubated at 37 °C. B. anthracis shows the characteristic shiny, rough with ground-glass appearance compared to the white slimy and smooth colonies of B. endophyticus.
Figure S2. Phenotypic electron microscopic examination of the morphology of B. endophyticus strains after 24 h incubation on nutrient agar containing 0.8% sodium bicarbonate stained using copper sulphate.
Figure S3. Phylogenetic tree of 16S ribosomal RNA sequence of the Bacillus endophyticus 3618_1C, 3631_9D, 3617_2C and 3631_10C strains with related Bacillus species using maximum likelihood. Geobacillus thermoglucosidasius was used as an out-group. Bootstrap values > 60 are indicated at the internodes.
Table S1. Plasmid comparison of the four sequenced Bacillus endophyticus strains (3618_1C, 3631_9D, 3631_10C, 3617_2C) with B. endophyticus Hbe603 strain.