The genetic relationship between R. microplus and R. decoloratus ticks in South Africa and their population structure

Show simple item record Baron, Samantha Van der Merwe, Nicolaas Albertus (Albie) Maritz-Olivier, Christine 2018-09-21T10:01:30Z 2018-12
dc.description.abstract Rhipicephalus microplus and R. decoloratus are one-host ticks that preferentially feed on cattle. They are capable of transmitting various tick-borne pathogens which may be detrimental to the agricultural and livestock industry in South Africa. Previous studies have shown that R. microplus forms five lineages in the R. microplus complex, segregating into different geographical areas based on mitochondrial markers. This study examined the phylogenetic relationship within and between R. microplus and R. decoloratus using the nuclear internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome oxidase subunit I (COI) genes. The results showed that the nuclear ITS2 marker is informative for interspecific variation but lacks the resolution for intraspecific variation. Analysis of the mitochondrial COI gene revealed that R. microplus ticks from South Africa grouped into a clade comprised of ticks from Asia and South America. The population structure of these two tick species was also investigated using novel microsatellite markers. Population structure analyses revealed that both the R. microplus and R. decoloratus populations presented with two genetic clusters. Rhipicephalus microplus ticks from the Kwa-Zulu Natal (KZN) province belonged to cluster 1, and those from the Eastern Cape (EC) province predominantly grouped into cluster 2. No observable population structure was noted for R. decoloratus. The overlap of genetic clusters in both species could be attributed to inbreeding between the regions by unrestricted movement of cattle across provinces. Such movement promotes tick mobility, gene flow and the homogenisation of tick populations. en_ZA
dc.description.department Genetics en_ZA
dc.description.embargo 2019-12-01
dc.description.librarian hj2018 en_ZA
dc.description.sponsorship Gauteng Department of Agriculture and Rural Development, Zoetis South Africa Pty. Ltd., and the National Research Foundation, THRIP grant nr: 83890, to C Maritz-Olivier. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Baron, S., Van der Merwe, N.A. & Maritz-Olivier, C. 2018, 'The genetic relationship between R. microplus and R. decoloratus ticks in South Africa and their population structure', Molecular Phylogenetics and Evolution, vol. 129, pp. 60-69. en_ZA
dc.identifier.issn 1055-7903 (print)
dc.identifier.issn 1095-9513 (online)
dc.identifier.other 10.1016/j.ympev.2018.08.003
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2018 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Molecular Phylogenetics and Evolution. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Molecular Phylogenetics and Evolution, vol. 129, pp. 60-69, 2018. doi : 10.1016/j.ympev.2018.08.003. en_ZA
dc.subject Rhipicephalus microplus en_ZA
dc.subject Rhipicephalus decoloratus en_ZA
dc.subject Recombination en_ZA
dc.subject Phylogenetics en_ZA
dc.subject Population structure en_ZA
dc.subject Microsatellites en_ZA
dc.subject Cytochrome oxidase subunit I (COI) en_ZA
dc.subject Internal transcribed spacer 2 (ITS2) en_ZA
dc.title The genetic relationship between R. microplus and R. decoloratus ticks in South Africa and their population structure en_ZA
dc.type Postprint Article en_ZA

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