Antimutagenic constituents from Monanthotaxis caffra (Sond.) Verdc.

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dc.contributor.author Makhuvele, Rhulani
dc.contributor.author Foubert, Kenn
dc.contributor.author Pieters, Luc
dc.contributor.author Verschaeve, Luc
dc.contributor.author Elgorashi, Esam E.
dc.date.accessioned 2018-09-03T07:23:25Z
dc.date.issued 2018-07
dc.description.abstract OBJECTIVES : Monanthotaxis caffra (Sond.) Verdc. (Annonaceae) has been reported to possess antitumoural properties. Preliminary screening showed that the crude methanolic leaf extract had strong antimutagenic effects against aflatoxin B1‐induced mutagenicity. The aim of this study was to isolate and evaluate the antimutagenic properties of the active constituents from M. caffra. METHODS : Different chromatographic, spectroscopic and spectrometric techniques were used for the isolation and identification of the antimutagenic constituents. The antimutagenic effect of the extract and compounds was evaluated using Ames, Vitotox and Comet assays. KEY FINDINGS : Bioassay‐guided fractionation of the methanolic leaf extract yielded two antimutagenic compounds identified as (+)‐crotepoxide and 5,6‐diacetoxy1‐benzoyloxymethyl‐1,3‐cyclohexadiene. Crotepoxide had strong antimutagenicity in the Vitotox assay with an IC50 value of 131 μg/ml. 5,6‐Diacetoxy‐1‐benzoyloxymethyl‐1,3‐cyclohexadiene showed strong antimutagenic activity in the Ames assay with an IC50 value of 348.9 μg/plate and no antimutagenic activity in the Vitotox test. Furthermore, the compound was able to inhibit, block or prevent biotransformation of aflatoxin B1 by repressing the proteins involved in transcription. CONCLUSIONS : Crotepoxide and 5,6‐diacetoxy‐1‐benzoyloxymethyl‐1,3‐cyclohexadiene have the potential to mitigate the risks arising from consumption of aflatoxin B1‐contaminated food and feed. en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.embargo 2019-07-01
dc.description.librarian hj2018 en_ZA
dc.description.sponsorship The National Research Foundation under grants (CPRR 87746 and NRF/FWO 87964), Fonds Weten-schappelijk Onderzoek (FWO) under grant (G001014N), Flemish Interuniversity Council (VLIR) under grant (ZEIN2014Z184) and Agricultural Research Council (ARCOVI). en_ZA
dc.description.uri http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)2042-7158 en_ZA
dc.identifier.citation Makhuvele, R., Foubert, K., Apers, S. et al. 2018, 'Antimutagenic constituents from Monanthotaxis caffra (Sond.) Verdc.', Journal of Pharmacy and Pharmacology, vol. 70, no. 7, pp. 976-984. en_ZA
dc.identifier.issn 0022-3573 (print)
dc.identifier.issn 2042-7158 (online)
dc.identifier.other 10.1111/jphp.12918
dc.identifier.uri http://hdl.handle.net/2263/66425
dc.language.iso en en_ZA
dc.publisher Wiley en_ZA
dc.rights © 2018 Royal Pharmaceutical Society. This is the pre-peer reviewed version of the following article : 'Antimutagenic constituents from Monanthotaxis caffra (Sond.) Verdc.', Journal of Pharmacy and Pharmacology, vol. 70, no. 7, pp. 976-984, 2018, doi : 10.1111/jphp.12918. The definite version is available at : http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)2042-7158. en_ZA
dc.subject (+)-crotepoxide en_ZA
dc.subject 5,6-diacetoxy-1-benzoyloxymethyl-1, 3-cyclohexadiene en_ZA
dc.subject Aflatoxin B-1 en_ZA
dc.subject Antimutagenicity en_ZA
dc.subject Monanthotaxis caffra en_ZA
dc.subject Cells en_ZA
dc.subject Extracts en_ZA
dc.subject Prevention en_ZA
dc.subject Mycotoxins en_ZA
dc.subject Metabolism en_ZA
dc.subject Derivatives en_ZA
dc.subject Genotoxicity en_ZA
dc.subject Crotepoxide en_ZA
dc.subject Medicinal plants en_ZA
dc.title Antimutagenic constituents from Monanthotaxis caffra (Sond.) Verdc. en_ZA
dc.type Postprint Article en_ZA


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