Changes in African elephant (Loxodonta africana) faecal steroid concentrations post-defaecation

Show simple item record Webber, Judith T. Henley, Michelle D. Pretorius, Yolanda Somers, Michael J. Ganswindt, Andre 2018-08-30T09:58:45Z 2018-08-30T09:58:45Z 2018-06-12
dc.description.abstract BACKGROUND : Faecal hormone metabolite measurement is a widely used tool for monitoring reproductive function and response to stressors in wildlife. Despite many advantages of this technique, the delay between defaecation, sample collection and processing may influence steroid concentrations, as faecal bacterial enzymes can alter steroid composition post-defaecation. OBJECTIVES : This study investigated changes in faecal glucocorticoid (fGCM), androgen (fAM) and progestagen (fPM) metabolite concentrations in faeces of a male and female African elephant (Loxodonta africana) post-defaecation and the influence of different faeces-drying regimes. METHOD : Subsamples of fresh faeces were frozen after being dried in direct sun or shade for 6, 20, 24, 48 and 72 h and 7 and 34 days. A subset of samples for each sex was immediately frozen as controls. Faecal hormone metabolite concentrations were determined using enzyme immunoassays established for fGCM, fAM and fPM monitoring in male and female African elephants. RESULTS : Hormone metabolite concentrations of all three steroid classes were stable at first, but changed distinctively after 20 h post-defaecation, with fGCM concentrations decreasing over time and fPM and fAM concentrations steadily increasing. In freeze-dried faeces fGCM concentrations were significantly higher than respective concentrations in sun-dried material, which were in turn significantly higher than fGCM concentrations in shade-dried material. In contrast, fAM concentrations were significantly higher in sun- and shade-dried faeces compared to freeze-dried faeces. Higher fPM concentrations were also found in air-dried samples compared to lyophilised faeces, but the effect was only significant for sun-dried material. CONCLUSION : The revealed time restriction for collecting faecal material for hormone monitoring from elephants in the wild should be taken into account to assure reliable and comparable results. However, if logistics allow a timely collection, non-invasive hormone measurement remains a powerful and reliable approach to provide information about an elephant’s endocrine status. en_ZA
dc.description.department Animal and Wildlife Sciences en_ZA
dc.description.department Centre for Wildlife Management en_ZA
dc.description.department Mammal Research Institute en_ZA
dc.description.department Zoology and Entomology en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship Elephants Alive and the Eugene Marais Chair of Wildlife Management. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Webber, J.T, Henley, M.D, Pretorius, Y, Somers, M.J, Ganswindt, A. 2018, ‘Changes in African elephant (Loxodonta africana) faecal steroid concentrations post-defaecation’, Bothalia 48(2), a2312. en_ZA
dc.identifier.issn 0006-8241 (print)
dc.identifier.issn 2311-9284 (online)
dc.identifier.other 10.4102/abc.v48i2.2312
dc.language.iso en en_ZA
dc.publisher AOSIS Open Journals en_ZA
dc.rights © 2018. The Authors. Licensee: AOSIS OpenJournals. This article is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. en_ZA
dc.subject Elephants en_ZA
dc.subject Hormone metabolite en_ZA
dc.subject Wildlife en_ZA
dc.subject Faecal material en_ZA
dc.subject African elephant (Loxodonta africana) en_ZA
dc.subject Urinary en_ZA
dc.subject Patterns en_ZA
dc.subject Testosterone en_ZA
dc.subject Feces en_ZA
dc.subject Storage methods en_ZA
dc.subject Conservation biology en_ZA
dc.subject Glucocorticoid metabolites en_ZA
dc.subject Adrenocortical function en_ZA
dc.subject Noninvasive assessment en_ZA
dc.subject Faecal glucocorticoid metabolite (fGCM) en_ZA
dc.title Changes in African elephant (Loxodonta africana) faecal steroid concentrations post-defaecation en_ZA
dc.type Article en_ZA

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