Isolation, identification and characterisation of butyric acid degrading bacterium from pit latrine faecal sludge
Loading...
Date
Authors
Njalam'mano, John Bright Joseph
Chirwa, Evans M.N.
Journal Title
Journal ISSN
Volume Title
Publisher
The Italian Association of Chemical Engineering
Abstract
Butyric acid is one of the volatile organic compounds that contribute significantly to pit latrine offensive smell.
The objective of this work was to isolate, identify and characterize pure butyric acid-utilising bacteria from
enriched mixed cultures. A bacterial strain capable of utilising butyric acid as a sole carbon source was
isolated from the pit latrine faecal sludge. Based on the 16 Subunit ribosomal Ribo-Nucleic Acid (16S rRNA)
analysis, the strain was identified as Pseudomonas aeruginosa. The strain has the capability to utilise 1000
mg/Land 5000 mg/L of butyric acid in 27 h and 42 h, respectively. Further experiments showed that its cell
growth was optimal at pH 7 when incubated at 30 oC and 150 rpm. The bacterial strain utilises butyric acid
even in the presence of paracresol. The isolation of the strain from indigenous pit latrine faecal sludge showed
that there is significant potential for application of microbes in treating noxious smells from the pit latrines.
Description
Keywords
Butyric acid, Pit latrine, Bacteria, Pseudomonas aeruginosa, Sole carbon source, Pseudomonas aeruginosa, Mixed cultures, Faecal sludge, Degrading bacteria, Bacterial strains, 16S ribosomal RNA (16S rRNA), Volatile organic compound (VOC), Sewage treatment, RNA, Nucleic acids
Sustainable Development Goals
Citation
Njalam Mano J., Chirwa E., 2018, Isolation, identification and characterisation of butyric acid degrading bacterium
from pit latrine faecal sludge, Chemical Engineering Transactions, 64, 445-450 DOI: 10.3303/CET1864075.