dc.contributor.author |
Adebayo, Salmon A.
|
|
dc.contributor.author |
Steel, Helen C.
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|
dc.contributor.author |
Shai, Leshweni J.
|
|
dc.contributor.author |
Eloff, Jacobus Nicolaas
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|
dc.date.accessioned |
2018-07-20T07:54:50Z |
|
dc.date.available |
2018-07-20T07:54:50Z |
|
dc.date.issued |
2017 |
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dc.description.abstract |
Peltophorum africanum extracts have been shown to possess many important medicinal benefits, including anti-inflammatory and antiviral activities. However, the mechanism of action is poorly understood. The mechanism of anti-inflammatory action was determined by measuring the synthesis of cytokines in lipopolysaccharide (LPS) stimulated RAW 264.7 macrophage cells in vitro. Compound 1 (CP1), compound 2 (CP2), and fraction F3.3.0 (F3.3.0) significantly reduced the synthesis of interleukin 1β (IL-1β) from RAW 264.7 cells (1.18, 1.32, and 0.92 ng/mL), respectively. Similarly, CP1, CP2, and F3.3.0 inhibited the production of IL-2 and tumor necrosis factor-α (TNF-α) by RAW 264.7 cells (0.41, 0.60, 0.74 and 0.11, 0.27, 0.24 ng/mL, respectively. In addition, CP1 and CP2 had lower cytotoxicity toward RAW 264.7 cells, with CP2 indicating the lowest cytotoxicity (LD50 = 207.88 µg/mL). The mechanism of action was found to be via the inhibition of pro-inflammation cytokines (IL-1 β and TNF-α). This observation may support the use of P africanum to treat pain-related conditions. |
en_ZA |
dc.description.department |
Immunology |
en_ZA |
dc.description.librarian |
am2018 |
en_ZA |
dc.description.sponsorship |
The National Research Foundation of
South Africa through grant number SFP150708124126. |
en_ZA |
dc.description.uri |
http://journals.sagepub.com/home/chp |
en_ZA |
dc.identifier.citation |
Adebayo, S.A., Steel, H.C., Shai, L.J. & Eloff, J.N. 2017, 'Investigation of the mechanism of anti-inflammatory action and cytotoxicity of a semipurified fraction and isolated compounds from the leaf of Peltophorum africanum (Fabaceae)', Journal of Evidence-Based Complementary and Alternative Medicine, vol. 22, no. 4, pp. 840-845. |
en_ZA |
dc.identifier.issn |
2156-5872 (print) |
|
dc.identifier.issn |
2156-5899 (online) |
|
dc.identifier.other |
10.1177/2156587217717417 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/65814 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Sage |
en_ZA |
dc.rights |
© The Adebayo et al.: 2018. Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License. |
en_ZA |
dc.subject |
IL-2 |
en_ZA |
dc.subject |
RAW 264.7 |
en_ZA |
dc.subject |
Peltophorum africanum |
en_ZA |
dc.subject |
Cytokines |
en_ZA |
dc.subject |
Lipopolysaccharide (LPS) |
en_ZA |
dc.subject |
Anti-inflammatory action |
en_ZA |
dc.subject |
Cytotoxicity |
en_ZA |
dc.subject |
IL-1β |
en_ZA |
dc.subject |
TNF-α |
en_ZA |
dc.title |
Investigation of the mechanism of anti-inflammatory action and cytotoxicity of a semipurified fraction and isolated compounds from the leaf of Peltophorum africanum (Fabaceae) |
en_ZA |
dc.type |
Article |
en_ZA |