Mycobacterium komaniense sp. nov., a rapidly growing non-tuberculous Mycobacterium species detected in South Africa

Show simple item record Gcebe, Nomakorinte Rutten, Victor P.M.G. Gey van Pittius, Nicolaas Naicker, Brendon Michel, Anita Luise 2018-07-18T07:23:17Z 2018-05
dc.description.abstract Some species of non-tuberculous mycobacteria (NTM) have been reported to be opportunistic pathogens of animals and humans. Recently there has been an upsurge in the number of cases of NTM infections, such that some NTM species are now recognized as pathogens of humans and animals. From a veterinary point of view, the major significance of NTM is the cross-reactive immune response they elicit against Mycobacterium bovis antigens, leading to misdiagnosis of bovine tuberculosis. Four NTM isolates were detected from a bovine nasal swab, soil and water, during an NTM survey in South Africa. These were all found using 16S rRNA gene sequence analysis to be closely related to Mycobacterium moriokaense. The isolates were further characterised by sequence analysis of the partial fragments of hsp65, rpoB and sodA. The genome of the type strain was also elucidated. Gene (16S rRNA, hsp65, rpoB and sodA) and protein sequence data analysis of 6 kDa early secretory antigenic target (ESAT 6) and 10 kDa culture filtrate protein (CFP-10) revealed that these isolates belong to a unique Mycobacterium species. Differences in phenotypic and biochemical traits between the isolates and closely related species further supported that these isolates belong to novel Mycobacterium species. We proposed the name Mycobacterium komaniense sp. nov. for this new species. The type strain is GPK 1020T (=CIP 110823T=ATCC BAA-2758). en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.embargo 2019-05-01
dc.description.librarian hj2018 en_ZA
dc.description.sponsorship WOTRO Science for global development; grant number W01.65.321.00. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Gcebe, N., Rutten, V.P.M.G., Van Pittius, N.G. et al. 2018, 'Mycobacterium komaniense sp. Nov., a rapidly growing nontuberculous Mycobacterium species detected in South Africa', International Journal of Systematic and Evolutionary Microbiology, vol. 68, no. 5, pp. 1526-1532. en_ZA
dc.identifier.issn 1466-5026 (print)
dc.identifier.issn 1466-5034 (online)
dc.identifier.other 10.1099/ijsem.0.002707
dc.language.iso en en_ZA
dc.publisher Microbiology Society en_ZA
dc.rights © 2018 IUMS. This is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. This article appears in Journal of General Virology, vol. 68 no. 5, pp. 1526-1532, 2018. doi : 10.1099/ijsem.0.002707. en_ZA
dc.subject Non-tuberculous mycobacteria (NTM) en_ZA
dc.subject Early secretory antigenic target (ESAT 6) en_ZA
dc.subject Culture filtrate protein (CFP-10) en_ZA
dc.subject Mycobacterium komaniense sp.nov. en_ZA
dc.subject Deoxyribonucleic acid (DNA) en_ZA
dc.subject Sequence analysis en_ZA
dc.subject 16S rRNA gene en_ZA
dc.subject Ribosomal en_ZA
dc.subject Ribonucleic acid (RNA) en_ZA
dc.subject Phylogeny en_ZA
dc.subject Genes en_ZA
dc.subject Cattle en_ZA
dc.subject South Africa (SA) en_ZA
dc.subject Microbiology en_ZA
dc.subject Purification en_ZA
dc.subject Isolation en_ZA
dc.subject Genetics en_ZA
dc.subject DNA sequence en_ZA
dc.subject Bovine en_ZA
dc.subject Bacterium identification en_ZA
dc.subject Bacterial gene en_ZA
dc.subject Atypical Mycobacterium en_ZA
dc.subject Bacterial DNA en_ZA
dc.title Mycobacterium komaniense sp. nov., a rapidly growing non-tuberculous Mycobacterium species detected in South Africa en_ZA
dc.type Postprint Article en_ZA

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