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dc.contributor.author | Zwart, Lizahn![]() |
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dc.contributor.author | Berger, David Kenneth![]() |
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dc.contributor.author | Moleleki, Lucy Novungayo![]() |
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dc.contributor.author | Van der Merwe, Nicolaas Albertus (Albie)![]() |
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dc.contributor.author | Myburg, Alexander Andrew![]() |
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dc.contributor.author | Naidoo, Sanushka![]() |
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dc.date.accessioned | 2018-07-16T09:36:58Z | |
dc.date.available | 2018-07-16T09:36:58Z | |
dc.date.issued | 2017-03 | |
dc.description.abstract | Eucalyptus species are cultivated for forestry and are of economic importance. The fungal stem canker pathogen Chrysoporthe austroafricana causes disease of varying severity on E. grandis. The Eucalyptus grandis-Chrysoporthe austroafricana interaction has been established as a model system for studying Eucalyptus antifungal defence. Previous studies revealed that the phytohormone salicylic acid (SA) affects the levels of resistance in highly susceptible (ZG14) and moderately resistant (TAG5) clones. The aims of this study were to examine histochemical changes in response to wounding and inoculation as well as host responses at the protein level. The anatomy and histochemical changes induced by wounding and inoculation were similar between the clones, suggesting that anatomical differences do not underlie their different levels of resistance. Tyloses and gum-like substances were present after inoculation and wounding, but cell death occurred only after inoculation. Hyphae of C. austroafricana were observed inside dead and living cells, suggesting that the possibility of a hemibiotrophic interaction requires further investigation. Proteomics analysis revealed the possible involvement of proteins associated with cell death, SA signalling and systemic resistance. In combination with previous information, this study forms a basis for future functional characterisation of candidate genes involved in resistance of E. grandis to C. austroafricana. | en_ZA |
dc.description.department | Forestry and Agricultural Biotechnology Institute (FABI) | en_ZA |
dc.description.department | Genetics | en_ZA |
dc.description.department | Microbiology and Plant Pathology | en_ZA |
dc.description.department | Plant Production and Soil Science | en_ZA |
dc.description.librarian | am2018 | en_ZA |
dc.description.sponsorship | The Genomics Research Institute (GRI, University of Pretoria); the National Research Foundation Thuthuka programme (South Africa, grant number 87912) and the Innovation Doctoral Scholarship (grant number 95128); Mondi, Sappi, and the Technology and Human Resources for Industry Programme. | en_ZA |
dc.description.uri | http://www.nature.com/srep | en_ZA |
dc.identifier.citation | Zwart, L., Berger, D.K., Moleleki, L.N. et al. 2017, 'Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana', Scientific Reports, vol. 7, art. no. 45402, pp. 1-12. | en_ZA |
dc.identifier.issn | 2045-2322 (online) | |
dc.identifier.other | 10.1038/srep45402 | |
dc.identifier.uri | http://hdl.handle.net/2263/65740 | |
dc.language.iso | en | en_ZA |
dc.publisher | Nature Publishing Group | en_ZA |
dc.rights | © The Author(s) 2017. This work is licensed under a Creative Commons Attribution 4.0 International License. | en_ZA |
dc.subject | Chrysoporthe austroafricana | en_ZA |
dc.subject | Eucalyptus grandis | en_ZA |
dc.subject | Signalling | en_ZA |
dc.subject | Defence response | en_ZA |
dc.subject | Salicylic acid (SA) | en_ZA |
dc.subject | Histochemical changes | en_ZA |
dc.subject | Wounding | en_ZA |
dc.subject | Inoculation | en_ZA |
dc.title | Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana | en_ZA |
dc.type | Article | en_ZA |