Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana
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Date
Authors
Zwart, Lizahn
Berger, David Kenneth
Moleleki, Lucy Novungayo
Van der Merwe, Nicolaas Albertus (Albie)
Myburg, Alexander Andrew
Naidoo, Sanushka
Journal Title
Journal ISSN
Volume Title
Publisher
Nature Publishing Group
Abstract
Eucalyptus species are cultivated for forestry and are of economic importance. The fungal stem canker
pathogen Chrysoporthe austroafricana causes disease of varying severity on E. grandis. The Eucalyptus
grandis-Chrysoporthe austroafricana interaction has been established as a model system for studying
Eucalyptus antifungal defence. Previous studies revealed that the phytohormone salicylic acid (SA)
affects the levels of resistance in highly susceptible (ZG14) and moderately resistant (TAG5) clones. The
aims of this study were to examine histochemical changes in response to wounding and inoculation
as well as host responses at the protein level. The anatomy and histochemical changes induced by
wounding and inoculation were similar between the clones, suggesting that anatomical differences
do not underlie their different levels of resistance. Tyloses and gum-like substances were present after
inoculation and wounding, but cell death occurred only after inoculation. Hyphae of C. austroafricana
were observed inside dead and living cells, suggesting that the possibility of a hemibiotrophic
interaction requires further investigation. Proteomics analysis revealed the possible involvement of
proteins associated with cell death, SA signalling and systemic resistance. In combination with previous
information, this study forms a basis for future functional characterisation of candidate genes involved
in resistance of E. grandis to C. austroafricana.
Description
Keywords
Chrysoporthe austroafricana, Eucalyptus grandis, Signalling, Defence response, Salicylic acid (SA), Histochemical changes, Wounding, Inoculation
Sustainable Development Goals
Citation
Zwart, L., Berger, D.K., Moleleki, L.N. et al. 2017, 'Evidence for salicylic acid signalling and histological changes in the defence response of Eucalyptus grandis to Chrysoporthe austroafricana',
Scientific Reports, vol. 7, art. no. 45402, pp. 1-12.