Rapid urban expansion has led to an increase in carnivores that live close to human dominated environments. Some carnivore species have successfully adapted to these novel conditions and taken advantage of opportunities associated with human habitation. Whilst many studies have compared carnivores living in protected areas to those in an urban setting, few have looked at the relationships between carnivores and human habitation within protected areas. In this study, we examined the effects of human habitation on the diet of spotted hyaenas (Crocuta crocuta) in the Kruger National Park (KNP), South Africa. Our results suggested a limited effect of anthropogenic resources on spotted hyaena diet in the KNP. We found neither temporal nor spatial variation in the amount of, nor types of, anthropogenic material in spotted hyaena scats, despite observations of more road side litter close to large anthropogenic sites. We therefore suggest that anthropogenic resources may not have been utilised completely according to abundance. We encourage further research evaluating potential secondary effects of human activity and infrastructure on spotted hyaena diet and also stable isotope approaches that may provide further insights into the importance of anthropogenic food for spotted hyaenas inside the KNP.