Prevalence and detection of Trichomonas vaginalis in HIV-infected pregnant women

Show simple item record Price, Collin M. Peters, Remco P.H. Steyn, Janré Mudau, Maanda Olivier, Dawie De Vos, Lindsey Morikawa, Erika Kock, Marleen M. Medina-Marino, Andrew Klausner, Jeffrey D. 2018-06-05T11:00:37Z 2018-05
dc.description.abstract BACKGROUND : Trichomonas vaginalis is a sexually transmitted infection associated with increased transmission of HIV and significant adverse birth outcomes; culture and polymerase chain reaction (PCR) are commonly used in diagnosis. METHODS : Consenting HIV-infected pregnant women were recruited from clinics in South Africa and screened for T. vaginalis using PCR. Polymerase chain reaction-positive women provided an additional sample for culture. We compared T. vaginalis detection between PCR and culture, and investigated how PCR cycle threshold (Ct) values differ among culture results. RESULTS : A total of 359 women were enrolled and 76 (20%) tested T. vaginalis PCR positive. Cultures were obtained from 61 of the PCR-positive women, and 38 (62%) were culture positive. The median baseline Ct of the PCR-positive/culture-positive group was 22.6 versus 38.0 among those who were PCR positive/culture negative (P < 0.001). Culture-positive cases had lower Ct values (higher DNA load); a Ct value less than 30 predicted positivity with a sensitivity of 97% and a specificity of 96%. CONCLUSIONS : Culture was positive in roughly half of PCR-positive cases. The culture-negative cases had significantly higher Ct values, indicating a lower concentration of T. vaginalis DNA. A Ct value of 30 provides a reliable threshold for predicting culture positivity. The clinical significance of culture-negative infections detected by PCR is still unclear. en_ZA
dc.description.department Medical Microbiology en_ZA
dc.description.department School of Health Systems and Public Health (SHSPH) en_ZA
dc.description.embargo 2019-05-01
dc.description.librarian hj2018 en_ZA
dc.description.sponsorship The National Institutes of Health (Grant No. 1-R21-HD084274-01A1), the US President's Emergency Plan for AIDS Relief (Cooperative Agreement: AID-674-A-12-00017), and a travel grant from the UCLA Center for World Health. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Price, C.M., Peters, R.P.H., Steyn, J. et al. 2018, 'Prevalence and detection of Trichomonas vaginalis in HIV-infected pregnant women', Sexually Transmitted Diseases, vol. 45, no. 5, pp. 332-336. en_ZA
dc.identifier.issn 0148-5717 (print)
dc.identifier.issn 1537-4521 (online)
dc.identifier.other 10.1097/OLQ.0000000000000756
dc.language.iso en en_ZA
dc.publisher Lippincott, Williams and Wilkins en_ZA
dc.rights © 2017 American Sexually Transmitted Diseases Association All rights reserved. This is a non-final version of an article published in final form in Sexually Transmitted Diseases, vol. 45, no. 5, pp. 332-336, 2018. doi : 10.1097/OLQ.0000000000000756. en_ZA
dc.subject Trichomonas vaginalis en_ZA
dc.subject Sexually transmitted infection (STI) en_ZA
dc.subject Human immunodeficiency virus (HIV) en_ZA
dc.subject Birth outcomes en_ZA
dc.subject Culture en_ZA
dc.subject Polymerase chain reaction (PCR) en_ZA
dc.subject Pregnancy en_ZA
dc.subject Low birth weight (LBW) en_ZA
dc.subject Risk factors en_ZA
dc.title Prevalence and detection of Trichomonas vaginalis in HIV-infected pregnant women en_ZA
dc.type Postprint Article en_ZA

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