South African medicinal plant extracts active against influenza A virus

Show simple item record Mehrbod, Parvaneh Abdalla, Muna Ali Njoya, Emmanuel Mfotie Ahmed, Aroke Shahid Fotouhi, Fatemeh Farahmand, Behrokh Gado, Dorcas A. Tabatabaian, Mansoureh Fasanmi, Olubunmi Gabriel Eloff, Jacobus Nicolaas McGaw, Lyndy Joy Fasina, Folorunso Oludayo 2018-05-24T05:34:38Z 2018-05-24T05:34:38Z 2018-03-27
dc.description Additional file 1: Figure S1. Estimated Marginal Means of Log HA titer. This graph shows the Log HA titer levels analyzed by GLM. en_ZA
dc.description Additional file 2: Figure S2. Estimated Marginal Means of Log HA decrement. This graph shows the decrement levels in Log HA titers analyzed by GLM. en_ZA
dc.description Additional file 3: Figure S3. Estimated Marginal Means of cell viability. This graph shows the ODs of the cell viability test analyzed by GLM. en_ZA
dc.description Additional file 4: Figure S4. Estimated Marginal Means of percentage of protection. This graph shows the protection of the extracts on the cell viability analyzed by GLM. en_ZA
dc.description.abstract BACKGROUND : Influenza infection remains a major health threat for animals and humans which crucially requires effective antiviral remedies. The usage of herbal medications as readily available alternatives for their compatibility with the body and fewer side effects compared to synthetic chemical treatments has become popular globally. The aim of this study was to investigate and screen in vitro anti-influenza activity of extracts of five South African medicinal plants, namely Tabernaemontana ventricosa, Cussonia spicata, Rapanea melanophloeos, Pittosporum viridiflorum and Clerodendrum glabrum, species which are used traditionally for the treatment of several diseases such as inflammatory and respiratory diseases. METHODS : Methanol, ethanol (100% and 30%), acetone, hot and cold water extracts of the powdered plants leaves were obtained by standard methods. The cytotoxicity was determined by the MTT colorimetric assay on MDCK cells. The concentrations below CC50 values were tested for antiviral activity against influenza A virus (IAV) in different combination treatments. The effect of extracts on viral surface glycoproteins and viral titer were tested by HI and HA virological assays, respectively. RESULTS : Based on the applied methods, the most effective results against IAV were obtained from Rapanea melanophloeos methanol leaf extract (EC50 = 113.3 μg/ml) and Pittosporum viridiflorum methanol, 100% and 30% ethanol and acetone leaf extracts (EC50 values = 3.6, 3.4, 19.2, 82.3 μg/ml, respectively) in all types of combined treatments especially in pre- and post-penetration combined treatments with highly significant effects against viral titer (P ≤ 0.01). CONCLUSION : The outcomes offer for the first time a scientific basis for the use of extracts of Rapanea melanophloeos and Pittosporum viridiflorum against IAV. It is worth focusing on the isolation and identification of effective active compounds and elucidating the mechanism of action from these species. However, Tabernaemontana ventricosa, Cussonia spicata and Clerodendrum glabrum leaf extracts were ineffective in vitro in this study. en_ZA
dc.description.department Paraclinical Sciences en_ZA
dc.description.department Production Animal Studies en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship This study was supported by the National Research Foundation under the cost center number N00882, project 98033RUN, South Africa. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Mehrbod, P., Abdalla, M.A., Njoya, E.M. et al. 2018, 'South African medicinal plant extracts active against influenza A virus', BMC Complementary and Alternative Medicine, vol. 18, art. no. 112, pp. 1-10. en_ZA
dc.identifier.issn 1472-6882 (online)
dc.identifier.other 10.1186/s12906-018-2184-y
dc.language.iso en en_ZA
dc.publisher BioMed Central en_ZA
dc.rights © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License. en_ZA
dc.subject Potential medicinal plants en_ZA
dc.subject Influenza A virus en_ZA
dc.subject Antiviral activity en_ZA
dc.subject Rapanea melanophloeos en_ZA
dc.subject Pittosporum viridiflorum en_ZA
dc.subject South Africa (SA) en_ZA
dc.subject Pandemic influenza en_ZA
dc.subject Pathogenesis en_ZA
dc.title South African medicinal plant extracts active against influenza A virus en_ZA
dc.type Article en_ZA

Files in this item

This item appears in the following Collection(s)

Show simple item record