p16 and Ki-67 immunohistochemical staining reduces inter- and intra-observer variability in the grading of cervical squamous intraepithelial lesions of South African women

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dc.contributor.author Solomon, Christa
dc.contributor.author Louw, Melanie
dc.contributor.author Van Aardt, M.C. (Matthys Cornelis)
dc.contributor.author Dreyer, Greta
dc.date.accessioned 2018-05-14T08:44:04Z
dc.date.available 2018-05-14T08:44:04Z
dc.date.issued 2017
dc.description.abstract BACKGROUND : Cervical carcinoma was the second leading malignancy in South African women (following breast carcinoma) in 2010. This study aimed to correlate histopathological criteria and immunohistochemical stains in terms of the grading of cervical intraepithelial precursor lesions and evaluate intra- and inter-observer variability with only histology and with additional immunohistochemical stains. METHODS : Archival tissue from large-loop excision of the transformation zone (LLETZ) was graded on two separate occasions by an independent observer in terms of lesional severity. The section with the highest grade precursor lesion was selected and submitted for immunohistochemical stains that included p16 and Ki-67. These stains were also evaluated on two separate occasions by an independent observer. RESULTS : This study showed kappa values of 0.47 and 0.46 respectively for the separate histological evaluations of the observer and the original pathology report. The kappa value for the two evaluations of the observer was 0.57. Thus inter- and intraobserver variability is fair with the use of routinely stained histological slides. The two Ki-67 assessments had a kappa value of 0.85 and the p16 had a value of 0.80. Intra-observer agreement was markedly higher when using immunohistochemistry. CONCLUSION : Although in most cases of precursor lesions of the cervix the grading can be made on routinely stained sections, intra- and inter-observer variability remains high. Immunohistochemical markers reduce this variability and aid in deciding in which group to place ambiguous lesions. en_ZA
dc.description.department Anatomical Pathology en_ZA
dc.description.department Obstetrics and Gynaecology en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship The authors would like to thank Prof Piet J. Bekker of the University of Pretoria and SA Medical Research Council for his expertise in the statistical analysis of this study.They would also like to thank the Struwig-Germeshuysen Cancer Research Trust for funding this study. The opinions voiced and conclusions drawn from this study are those of the researchers alone and are not necessarily supported by the Trust. en_ZA
dc.description.uri http://www.sajgo.co.za/index.php/sajgo en_ZA
dc.description.uri https://medpharm.tandfonline.com/toc/ojgo20/current en_ZA
dc.identifier.citation Solomon, C., Louw, M., Van Aardt, M.C. & Dreyer, G. 2017, 'p16 and Ki-67 immunohistochemical staining reduces inter- and intra-observer variability in the grading of cervical squamous intraepithelial lesions of South African women', Southern African Journal of Gynaecological Oncology, vol. 9, no. 2, pp. 25-29. en_ZA
dc.identifier.issn 2074-2835 (print)
dc.identifier.issn 2220-105X (online)
dc.identifier.other 10.1080/20742835.2017.1370841
dc.identifier.uri http://hdl.handle.net/2263/64934
dc.language.iso en en_ZA
dc.publisher Medpharm Publications, NISC (Pty) Ltd and Informa UK Limited [trading as the Taylor & Francis Group] en_ZA
dc.rights © 2017 The Author(s). Open Access article distributed under the terms of the Creative Commons License [CC BY-NC 3.0]. en_ZA
dc.subject Observer variability en_ZA
dc.subject Large-loop excision of the transformation zone (LLETZ) en_ZA
dc.subject South Africa (SA) en_ZA
dc.subject Cervical intraepithelial neoplasia (CIN) en_ZA
dc.subject Human papillomavirus (HPV) en_ZA
dc.subject South African women en_ZA
dc.subject.other Health sciences article SDG-03
dc.subject.other SDG-03: Good health and well-being
dc.title p16 and Ki-67 immunohistochemical staining reduces inter- and intra-observer variability in the grading of cervical squamous intraepithelial lesions of South African women en_ZA
dc.type Article en_ZA


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