Anti-HIV activity of ocimum labiatum extract and isolated pheophytin-a

Show simple item record Kapewangolo, Petrina T. Kandawa-Schulz, Martha Meyer, Debra 2018-04-10T08:24:50Z 2018-04-10T08:24:50Z 2017-11-06
dc.description.abstract Ocimum plants are traditionally used to manage HIV/AIDS in various African countries. The effects of Ocimum labiatum extract on HIV-1 protease (PR) and reverse transcriptase (RT) is presented here along with characterization of an identified bioactive compound, achieved through 1H- and 13C-NMR. The extract’s effect on HIV-1 replication was assessed by HIV-1 p24 antigen capture. Cytotoxicity of samples was evaluated using tetrazolium dyes and real-time cell electronic sensing (RT-CES). Ocimum labiatum inhibited HIV-1 PR with an IC50 value of 49.8 0.4 g/mL and presented weak inhibition (21%) against HIV-1 RT. The extract also reduced HIV-1 replication in U1 cells at a non-cytotoxic concentration (25 g/mL). The CC50 value of the extract in U1 cells was 42.0 0.13 g/mL. The HIV-1 PR inhibiting fraction was purified using prep-HPLC and yielded a chlorophyll derivative, pheophytin-a (phy-a). Phy-a inhibited HIV-1 PR with an IC50 value of 44.4 1.5 g/mL (51 1.7 M). The low cytotoxicity of phy-a in TZM-bl cells was detected by RT-CES and the CC50 value in U1 cells was 51.3 1.0 g/mL (58.9 1.2 M). This study provides the first in vitro evidence of anti-HIV activity of O. labiatum and isolated phy-a, supporting further investigation of O. labiatum for lead compounds against HIV-1. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship Southern African Biochemistry and Informatics for Natural Products (SABINA), Ministry of Education (Namibia), the Technology Innovation Agency (TIA, RSA), Margaret McNamara Memorial Fund (MMMF) and the University of Pretoria. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Kapewangolo, P., Kandawa-Schulz, M. & Meyer, D. 2017, 'Anti-HIV activity of ocimum labiatum extract and isolated pheophytin-a', Molecules, vol. 22, no. 11, art. no. 1763, pp. 1-12. en_ZA
dc.identifier.issn 1420-3049 (online)
dc.identifier.other 10.3390/molecules22111763
dc.language.iso en en_ZA
dc.publisher MDPI Publishing en_ZA
dc.rights © 2017 by The Authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license ( en_ZA
dc.subject Ocimum labiatum en_ZA
dc.subject Pheophytin-a en_ZA
dc.subject HIV-1 protease en_ZA
dc.subject HIV-1 reverse transcriptase en_ZA
dc.subject HIV-1 replication en_ZA
dc.subject Human immunodeficiency virus (HIV) en_ZA
dc.subject Acquired immune deficiency syndrome (AIDS) en_ZA
dc.subject Reverse transcriptase (RT) en_ZA
dc.subject Real-time cell electronic sensing (RT-CES) en_ZA
dc.title Anti-HIV activity of ocimum labiatum extract and isolated pheophytin-a en_ZA
dc.type Article en_ZA

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