Genetic diversity and phylogenetic relationships of coevolving symbiont-harboring insect trypanosomatids, and their neotropical dispersal by invader African blowflies (Calliphoridae)

Show simple item record Borghesan, Tarcilla C. Campaner, Marta Matsumoto, Tania E. Espinosa, Omar A. Razafindranaivo, Victor Paiva, Fernando Carranza, Julio C. Anez, Nestor Neves, L.C.B.G.D. (Luís) Teixeira, Marta M. G. Camargo, Erney P. 2018-03-12T07:20:55Z 2018-03-12T07:20:55Z 2018-02-07
dc.description.abstract This study is about the inter- and intra-specific genetic diversity of trypanosomatids of the genus Angomonas, and their association with Calliphoridae (blowflies) in Neotropical and Afrotropical regions. Microscopic examination of 3,900 flies of various families, mostly Calliphoridae, revealed that 31% of them harbored trypanosomatids. Small subunit rRNA (SSU rRNA) barcoding showed that Angomonas predominated (46%) over the other common trypanosomatids of blowflies of genera Herpetomonas and Wallacemonas. Among Angomonas spp., A. deanei was much more common than the two-other species, A. desouzai and A. ambiguus. Phylogenetic analyses based on SSU rRNA, glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) and internal transcribed spacer rDNA (ITS rDNA) sequences revealed a marked genetic diversity within A. deanei, which comprised four infraspecific genotypes (Dea1– Dea4), and four corresponding symbiont genotypes (Kcr1–Kcr4). Host and symbiont phylogenies were highly congruent corroborating their co-divergence, consistent with host-symbiont interdependent metabolism and symbiont reduced genomes shaped by a long coevolutionary history. We compared the diversity of Angomonas/symbionts from three genera of blowflies, Lucilia, Chrysomya and Cochliomyia. A. deanei, A. desouzai, and A. ambiguus were found in the three genera of blowflies in South America. In Africa, A. deanei and A. ambiguus were identified in Chrysomya. The absence of A. desouzai in Africa and its presence in Neotropical Cochliomyia and Lucilia suggests parasite spillback of A. desouzai into Chrysomya, which was most likely introduced four decades ago from Africa into the Neotropic. The absence of correlation between parasite diversity and geographic and genetic distances, with identical genotypes of A. deanei found in the Neotropic and Afrotropic, is consistent with disjunct distribution due to the recent human-mediated transoceanic dispersal of Angomonas by Chrysomya. This study provides the most comprehensive data gathered so far on the genetic repertoires of a genus of trypanosomatids found in flies from a wide geographical range. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2018 en_ZA
dc.description.sponsorship The PROAFRICA, INCT-EPIAMO, and PROSUL programs of CNPq, PNIPB of Capes, and FAPESP (Process 2016/07487-0). CAPEs (PNPD) granted a postdoctoral scholarship to TB. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Borghesan TC, Campaner M, Matsumoto TE, Espinosa OA, Razafindranaivo V, Paiva F, Carranza JC, Añez N, Neves L, Teixeira MMG and Camargo EP (2018) Genetic Diversity and Phylogenetic Relationships of Coevolving Symbiont-Harboring Insect Trypanosomatids, and Their Neotropical Dispersal by Invader African Blowflies (Calliphoridae). Front. Microbiol. 9:131. DOI: 10.3389/fmicb.2018.00131. en_ZA
dc.identifier.issn 1664-302X (online)
dc.identifier.other 10.3389/fmicb.2018.00131
dc.language.iso en en_ZA
dc.publisher Frontiers Media en_ZA
dc.rights © 2018 Borghesan, Campaner, Matsumoto, Espinosa, Razafindranaivo, Paiva, Carranza, Añez, Neves, Teixeira and Camargo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). en_ZA
dc.subject Endosymbiont en_ZA
dc.subject Reduced genomes en_ZA
dc.subject Fly parasites en_ZA
dc.subject Transoceanic dispersal en_ZA
dc.subject Co-evolution en_ZA
dc.subject Phylogeography en_ZA
dc.subject Parasite spillover en_ZA
dc.subject DNA barcoding en_ZA
dc.subject African blowflies (Calliphoridae)
dc.title Genetic diversity and phylogenetic relationships of coevolving symbiont-harboring insect trypanosomatids, and their neotropical dispersal by invader African blowflies (Calliphoridae) en_ZA
dc.type Article en_ZA

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