dc.contributor.author |
Strydom, Elrea
|
|
dc.contributor.author |
Pietersen, Gerhard
|
|
dc.date.accessioned |
2018-02-14T07:28:34Z |
|
dc.date.issued |
2018-05 |
|
dc.description.abstract |
Infection of soybean by the plant cytorhabdovirus soybean blotchy mosaic virus (SbBMV) results in significant yield losses in the temperate, lower-lying soybean production regions of South Africa. A 277 bp portion of the RNA-dependent RNA polymerase gene of 66 SbBMV isolates from different: hosts, geographical locations in South Africa, and times of collection (spanning 16 years) were amplified by RT-PCR and sequenced to investigate the genetic diversity of isolates. Phylogenetic reconstruction revealed three main lineages, designated Groups A, B and C, with isolates grouping primarily according to geographic origin. Pairwise nucleotide identities ranged between 85.7% and 100% among all isolates, with isolates in Group A exhibiting the highest degree of sequence identity, and isolates of Groups A and B being more closely related to each other than to those in Group C. This is the first study investigating the genetic diversity of SbBMV. |
en_ZA |
dc.description.department |
Forestry and Agricultural Biotechnology Institute (FABI) |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.embargo |
2019-05-01 |
|
dc.description.librarian |
hj2018 |
en_ZA |
dc.description.sponsorship |
The Association of African Universities (AAU), the Genomics Research Institute (GRI) at the University of Pretoria, South Africa and through the National Research Foundation Incentive Grant for Rated Scientists. |
en_ZA |
dc.description.uri |
http://link.springer.com/journal/705 |
en_ZA |
dc.identifier.citation |
Strydom, E. & Pietersen, G. Diversity of partial RNA-dependent RNA polymerase gene sequences of soybean blotchy mosaic virus isolates from different host-, geographical- and temporal origins. Archives of Virology (2018) 163: 1299-1305. https://doi.org/10.1007/s00705-018-3722-0. |
en_ZA |
dc.identifier.issn |
0304-8608 (print) |
|
dc.identifier.issn |
1432-8798 (online) |
|
dc.identifier.other |
10.1007/s00705-018-3722-0 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/63948 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Springer |
en_ZA |
dc.rights |
© Springer-Verlag GmbH Austria, part of Springer Nature 2018. The original publication is available at : http://link.springer.comjournal/705. |
en_ZA |
dc.subject |
Soybean blotchy mosaic virus (SbBMV) |
en_ZA |
dc.subject |
RNA-dependent RNA polymerase gene |
en_ZA |
dc.subject |
Maximum-likelihood analysis |
en_ZA |
dc.subject |
Pairwise nucleotide similarity |
en_ZA |
dc.title |
Diversity of partial RNA-dependent RNA polymerase gene sequences of soybean blotchy mosaic virus isolates from different host-, geographical- and temporal origins |
en_ZA |
dc.type |
Postprint Article |
en_ZA |