Cigarette smoke condensate attenuates phorbol ester-mediated neutrophil extracellular trap formation

Show simple item record Bokaba, Refilwe Philadelphia Anderson, Ronald Theron, Annette J. Tintinger, Gregory Ronald 2018-01-26T07:31:34Z 2018-01-26T07:31:34Z 2017-09
dc.description.abstract BACKGROUND : Neutrophil extracellular traps (NETs) constitute a network of chromatin fibres containing histone and antimicrobial peptides that are released by activated neutrophils. NETs protect the host against infection by trapping and facilitating phagocytosis of potentially harmful pathogens. OBJECTIVES : The aim of the current study was to investigate the effects of cigarette smoke condensate (CSC) on phorbol-ester (PMA)-mediated NETosis in vitro. METHODS : Isolated human blood neutrophils were exposed to PMA (6.25 ng/ml) in the presence or absence of CSC (40-80 μg/ ml) for 90 min at 37oC. NET formation was measured using a spectrofluorimetric procedure to detect extracellular DNA and fluorescence microscopy was used to visualize nets. Oxygen consumption by PMA-activated neutrophils was measured using an oxygen sensitive electrode. RESULTS : Activation of neutrophils with PMA was associated with induction of NETosis that was significantly attenuated in the presence of CSC (40 and 80 μg/ml), with mean fluorescence intensities of 65% and 66% of that observed with untreated cells, respectively, and confirmed by fluorescence microscopy. The rate and magnitude of oxygen consumption by activated neutrophils pre-treated with CSC (80 μg/ml) was significantly less than that observed with untreated cells (73% of the control system), indicative of decreased production of reactive oxidants in the presence of CSC. CONCLUSION : The inhibition of NETosis observed in the presence of CSC correlated with attenuation of oxygen consumption by PMA-activated neutrophils suggesting a mechanistic relationship between these events. If operative in vivo, smoking-related attenuation of NETosis may impair host immune responses and increase the risk of respiratory infections. en_ZA
dc.description.department Immunology en_ZA
dc.description.department Internal Medicine en_ZA
dc.description.librarian am2018 en_ZA
dc.description.uri en_ZA
dc.identifier.citation Bokaba RP, Anderson R, Theron AJ, Tintinger GR. Cigarette smoke condensate attenuates phorbol ester-mediated neutrophil extracellular trap formation. Afri Health Sci. 2017;17(3): 896-904. 10.4314/ahs.v17i3.33. en_ZA
dc.identifier.issn 1680-6905 (print)
dc.identifier.issn 1729-0503 (online)
dc.identifier.other 10.4314/ahs.v17i3.33
dc.language.iso en en_ZA
dc.publisher Makerere University Medical School en_ZA
dc.rights © 2017 Bokaba et al; licensee African Health Sciences. This is an Open Access article distributed under the termsof the Creative commons Attribution License ( en_ZA
dc.subject Neutrophils en_ZA
dc.subject Reactive oxygen species en_ZA
dc.subject Respiratory infection en_ZA
dc.subject Smoking en_ZA
dc.subject Systems en_ZA
dc.subject Netosis en_ZA
dc.subject Disease en_ZA
dc.subject Bacteria en_ZA
dc.subject Thrombosis en_ZA
dc.subject Susceptibility en_ZA
dc.subject Mechanisms en_ZA
dc.subject Free radicals en_ZA
dc.title Cigarette smoke condensate attenuates phorbol ester-mediated neutrophil extracellular trap formation en_ZA
dc.type Article en_ZA

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