dc.contributor.author |
Sungirai, Marvelous
|
|
dc.contributor.author |
Baron, Samantha
|
|
dc.contributor.author |
Moyo, Doreen Zandile
|
|
dc.contributor.author |
De Clercq, Patrick
|
|
dc.contributor.author |
Maritz-Olivier, Christine
|
|
dc.contributor.author |
Madder, Maxime
|
|
dc.date.accessioned |
2017-11-28T08:13:30Z |
|
dc.date.issued |
2018-01 |
|
dc.description.abstract |
Acaricide resistance is one of the greatest threats towards the successful control of vector ticks worldwide. Communal farmers of Zimbabwe use amitraz as the most common acaricide with occasional usage of pyrethroids and organophosphates. As a strategy towards developing an effective acaricide resistance management system in Zimbabwe, screening was done by genotyping Rhipicephalus microplus tick populations using molecular markers associated with resistance to these chemicals. The frequency of the mutant allele for the octopamine/tyramine receptor marker associated with amitraz resistance was high (0.55) and a large proportion 78.5% (288/367) of heterozygote genotypes were observed indicating balancing selection. Of the communal dipping tanks where R. microplus occurred 37.8% (39/103) showed complete resistance genotypes for amitraz. The carboxylesterase marker that has been associated with resistance to organophosphate and pyrethroids indicated no selection pressure in these chemical groups with a low frequency (0.052) of the mutant allele and 89.6% (329/367) sampled ticks showing homozygous susceptibility genotypes. Heterozygous genotypes were present at 27.2% (28/103) of the dipping tanks. The L641 mutation in the voltage–gated sodium channel gene associated with pyrethroid resistance was not detected in Zimbabwean samples. This would suggest a different mechanism of resistance to pyrethroids in these tick populations. Sequence analysis of the octopamine/tyramine receptor gene revealed the presence of other mutations in this region, it will be important to investigate their association with amitraz resistance. These results present the first molecular genotyping of resistance profiles of R. microplus tick populations from Zimbabwe. |
en_ZA |
dc.description.department |
Genetics |
en_ZA |
dc.description.department |
Veterinary Tropical Diseases |
en_ZA |
dc.description.embargo |
2019-01-01 |
|
dc.description.librarian |
hj2017 |
en_ZA |
dc.description.sponsorship |
The Belgium Department of Development Co-operation (DGD) |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/ttbdis |
en_ZA |
dc.identifier.citation |
Sungirai, M., Baron, S., Moyo, D.Z., De Clercq, P., Maritz-Olivier, C. & Madder, M. 2018, 'Genotyping acaricide resistance profiles of Rhipicephalus microplus tick populations from communal land areas of Zimbabwe', Ticks and Tick-borne Diseases, vol. 9, no. 1, pp. 2-9. |
en_ZA |
dc.identifier.issn |
1877-959X ( print) |
|
dc.identifier.issn |
1877-9603 (online) |
|
dc.identifier.other |
10.1016/j.ttbdis.2017.10.017 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/63371 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2017 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Ticks and Tick-borne Diseases. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Ticks and Tick-borne Diseases, vol. 9, no. 1, pp. 2-9, 2018. doi : 10.1016/j.ttbdis.2017.10.017. |
en_ZA |
dc.subject |
Rhipicephalus microplus |
en_ZA |
dc.subject |
Genotyping |
en_ZA |
dc.subject |
Acaricide resistance |
en_ZA |
dc.subject |
Amitraz |
en_ZA |
dc.subject |
Zimbabwe |
en_ZA |
dc.subject |
Susceptibility |
en_ZA |
dc.subject |
Gene sequence |
en_ZA |
dc.subject |
Gene mutation |
en_ZA |
dc.subject |
Gene frequency |
en_ZA |
dc.subject |
Drug resistance |
en_ZA |
dc.subject |
Balancing selection |
en_ZA |
dc.subject |
Carboxylesterase marker |
en_ZA |
dc.subject |
Octopamine/tyramine receptor |
en_ZA |
dc.subject |
Voltage-gated sodium channel |
en_ZA |
dc.subject.other |
Veterinary science articles SDG-01 |
en_ZA |
dc.subject.other |
SDG-01: No poverty |
|
dc.title |
Genotyping acaricide resistance profiles of Rhipicephalus microplus tick populations from communal land areas of Zimbabwe |
en_ZA |
dc.type |
Postprint Article |
en_ZA |