Anti-bacterial, free radical scavenging activity and cytotoxicity of acetone extracts of Grewia flava
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Date
Authors
Lamola, Stella Makgabo
Dzoyem, Jean Paul
Botha, Francien Susanna
Van Wyk, Candice
Journal Title
Journal ISSN
Volume Title
Publisher
Kampala, Uganda : Faculty of Medicine, Makerere University
Abstract
BACKGROUND : Bacterial infections of the gastrointestinal tract (GIT) cause vomiting, diarrhoea and even systemic disease. There
is a need for the development of natural products into alternative and safer medicines.
OBJECTIVES : This study evaluated the anti-microbial activity of extracts prepared from berries, leaves,bark and roots of the edible
plant Grewia flava.
METHODS : The anti-bacterial activity was evaluated by the broth microdilution method. Anti-oxidant activity of the most active
extracts was performed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The cytotoxicity of the extracts was determined using
the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
RESULTS : The acetone extracts of the leaves and roots showed the best activity with MIC values as low as 0.03 mg/mL against
Staphylococcus aureus and Salmonella typhimurium and 0.07 mg/mL against Bacillus cereus, Escherichia coli and Staphylococcus aureus.
Quantitative analysis of the scavenging ability showed that acetone extracts exhibited good free radical scavenging activity in a
dose-dependent manner. The berries extract had the highest LC50 (lowest toxicity) of 551.68 68 μg/mL.
CONCLUSION : Acetone extract of leaves and roots of Grewia flava contain anti-microbial and anti-oxidant compounds and could
therefore be used as a natural product with little toxicity to host cells.
Description
Keywords
Extracts, Anti-microbial, Cytotoxicity, Enteric pathogens, Grewia flava, Medicinal plants, Asiatica L., Combretaceae, Gastrointestinal tract (GIT)
Sustainable Development Goals
Citation
Lamola SM, Dzoyem JP, Botha F, Wyk Cv. Antibacterial, free radical scavenging activity and cytotoxicity of acetone extracts of Grewiaflava.
Afri Health Sci. 2017;17(3): 790-796. https://dx.DOI.org/ 10.4314/ahs.v17i3.22.