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dc.contributor.author | NandaKafle, Gitanjali![]() |
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dc.contributor.author | Seale, Tarren![]() |
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dc.contributor.author | Flint, Toby![]() |
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dc.contributor.author | Nepal, Madhav![]() |
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dc.contributor.author | Venter, S.N. (Stephanus Nicolaas)![]() |
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dc.contributor.author | Brözel, Volker Siegfried![]() |
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dc.date.accessioned | 2017-11-17T07:54:06Z | |
dc.date.available | 2017-11-17T07:54:06Z | |
dc.date.issued | 2017-07-27 | |
dc.description.abstract | Escherichia coli is widely considered to not survive for extended periods outside the intestines of warm-blooded animals; however, recent studies demonstrated that E. coli strains maintain populations in soil and water without any known fecal contamination. The objective of this study was to investigate whether the niche partitioning of E. coli occurs between cattle and their pasture. We attempted to clarify whether E. coli from bovine feces differs phenotypically and genotypically from isolates maintaining a population in pasture soil over winter. Soil, bovine fecal, and run-off samples were collected before and after the introduction of cattle to the pasture. Isolates (363) were genotyped by uidA and mutS sequences and phylogrouping, and evaluated for curli formation (Rough, Dry, And Red, or RDAR). Three types of clusters emerged, viz. bovine-associated, clusters devoid of cattle isolates and representing isolates endemic to the pasture environment, and clusters with both. All isolates clustered with strains of E. coli sensu stricto, distinct from the cryptic species Clades I, III, IV, and V. Pasture soil endemic and bovine fecal populations had very different phylogroup distributions, indicating niche partitioning. The soil endemic population was largely comprised of phylogroup B1 and had a higher average RDAR score than other isolates. These results indicate the existence of environmental E. coli strains that are phylogenetically distinct from bovine fecal isolates, and that have the ability to maintain populations in the soil environment. | en_ZA |
dc.description.department | Microbiology and Plant Pathology | en_ZA |
dc.description.librarian | am2017 | en_ZA |
dc.description.sponsorship | This research was funded by grant SA1200560 from USDA/NRCS/CIG. GN was supported by a fellowship from the South Dakota Agricultural Experiment Station. We thank Dr Alexander (Sandy) Smart for access to the grazing plots and Ben Benson for assistance with constructing phylogenetic trees. We also thank Dr. Seth Walk, Montana State University, for donating Clade isolates. We acknowledge the use of the SDSU- Functional Genomics Core Facility, supported by NSF/EPSCoR Grant No. 0091948, the South Dakota 2010 Drought Initiative, and the South Dakota Agricultural Experiment Station. | en_ZA |
dc.description.uri | http://www.sherpa.ac.uk/romeo/issn/1342-6311/ | en_ZA |
dc.identifier.citation | NandaKafle, G., Seale, T., Flint, T., Nepal, M., Venter, S.N. & Brozel, V.S. 2017, 'Distribution of diverse Escherichia coli between cattle and pasture', Microbes and Environments, vol. 32, no. 3, pp. 226-233. | en_ZA |
dc.identifier.issn | 1342-6311 (print) | |
dc.identifier.issn | 1347-4405 (online) | |
dc.identifier.other | 10.1264/jsme2.ME17030 | |
dc.identifier.uri | http://hdl.handle.net/2263/63191 | |
dc.language.iso | en | en_ZA |
dc.publisher | The Japanese Society of Microbial Ecology | en_ZA |
dc.rights | © 2017 Japanese Society of Microbial Ecology, The Japanese Society of Soil Microbiology, Taiwan Society of Microbial Ecology, and Japanese Society of Plant Microbe Interactions. Article is published in an open-access journal. | en_ZA |
dc.subject | Escherichia coli | en_ZA |
dc.subject | Niche partitioning | en_ZA |
dc.subject | Pasture | en_ZA |
dc.subject | Bovine feces | en_ZA |
dc.subject | Cattle | en_ZA |
dc.subject | Phylogroup | en_ZA |
dc.subject | Long-term survival | en_ZA |
dc.subject | Temperate soils | en_ZA |
dc.subject | Host specificity | en_ZA |
dc.subject | Bacillus subtilis | en_ZA |
dc.subject | Phylogenetic analysis | en_ZA |
dc.subject | Subtropical environment | en_ZA |
dc.subject | Beach sand | en_ZA |
dc.subject | Lake Superior | en_ZA |
dc.title | Distribution of diverse Escherichia coli between cattle and pasture | en_ZA |
dc.type | Article | en_ZA |