Cowdria ruminantium: stability and preservation of the organism

Show simple item record Logan, L.L.
dc.contributor.editor Bigalke, R.D. 2017-11-13T09:22:01Z 2017-11-13T09:22:01Z 2017 1987
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en_ZA
dc.description.abstract Blood collected in either sodium heparin or disodium edetate vacutainers from febrile goats infected with 4 isolates of Cowdria ruminantium and cryopreserved with 10% dimethyl sulphoxide at -70⁰C and -196⁰C was an effective stabilate to initiate heartwater infections in goats. A homogenized pool of whole Amblyomma variegatum ticks in Snyder's buffer, maintained at -196⁰C, was used to infect a goat with C. ruminantium. Liver and spleen collected from Swiss mice infected with the Kwanyanga isolate of C. ruminantium were homogenized in Snyder's buffer, maintained at -196⁰C and were used to initiate infections in mice. Fresh blood collected from febrile goats and maintained at 4⁰C for as long as 72 h was infectious to mice. Neutrophils separated from blood of C. ruminantium infected goats and maintained in modified RPMI medium at 37⁰C for 68 h were infectious for a goat. Similarly neutrophils from a 2nd infected goat maintained for 96 h at 37⁰C were infectious for mice. en_ZA
dc.identifier.citation Logan, LL 1987, 'Cowdria ruminantium: stability and preservation of the organism', Onderstepoort Journal of Veterinary Research, vol. 54, no. 3, pp. 187-191. en_ZA
dc.identifier.issn 0330-2465
dc.language.iso en en_ZA
dc.publisher Published by the Government Printer, Pretoria. en_ZA
dc.rights ©ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). ©University of Pretoria. Dept. of Library Services (digital). en_ZA
dc.subject Veterinary medicine en_ZA
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Cowdria ruminantium: stability and preservation of the organism en_ZA
dc.title.alternative Heartwater : past, present and future : proceedings of a workshop held at Berg en Dal, Kruger National Park, on 8-16 September 1986 en_ZA
dc.type Article en_ZA

Files in this item

This item appears in the following Collection(s)

Show simple item record