Mycobacterium bovis infection in cattle at the wildlife/livestock interface in northern KwaZulu-Natal province, South Africa
Sichewo, P.R.; Etter, Eric M.C.; University of Pretoria. Faculty of Veterinary Science. Dept. of Veterinary Tropical Diseases; University of Pretoria. Faculty of Veterinary Science. Dept. of Production Animal Studies
A cross-sectional study was carried out in Northern KwaZulu-
Natal at the wildlife/livestock interface to determine the
prevalence of Mycobacterium bovis infection in cattle using
a modified BOVIGAM® interferon gamma assay (IFN-γ).
Although cattle are known as the primary host, M. bovis can
also affect other domestic animals, wildlife and humans.
Using a random sampling technique, whole blood samples
were collected from 387 cattle registered at two dip tanks
(Mpempe and Nkomo) in the uMkhanyakude district, which
consisted of 267 females and 120 males from a total of 100
herds. The apparent M. bovis prevalence rate at animal level
was 13.9% (95% confidence interval (CI) 10.6 - 17.4) and the
true prevalence indicated a similar prevalence rate of 13.6%
(95% CI 10.2 - 16.9). The apparent and true prevalence rate
at Nkomo dip tank was 7.9% (95% CI 4.4 - 13.6) and 5.7% (95% CI 1.4 - 11.6) respectively. The apparent and true prevalence
rates at Mpempe dip tank were almost similar: 17.3% (95%
CI 13.1 - 22.5) and 18% (95% CI 11.9 - 24.1) respectively. At
Mpepe dip tank 20/52 (39%) of the farmers had at least one
test positive animal, whilst at Nkomo dip tank, this figure was
8/48 (17%). Based on sex, 11 (9.1%, 95% CI 3.9 - 14.2) of the
males and 43 (16.1%, 95% CI 11.7 - 20.5) of the females tested
positive with the assay.
This study forms part of a One Health project at the wildlife/
livestock interface. Confirmation of infection will be done
through isolation and culture of tissues from test positive
animals. The isolates will be genotyped and compared with
isolates from wildlife, to monitor M. bovis transmission at the
Poster presented at the University of Pretoria, Faculty of Veterinary Science Faculty Day, September 07, 2017, Pretoria, South Africa.