Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems

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dc.contributor.author Guerrero, Leandro D.
dc.contributor.author Vikram, Surendra
dc.contributor.author Makhalanyane, Thulani P.
dc.contributor.author Cowan, Don A.
dc.date.accessioned 2017-10-17T10:59:45Z
dc.date.issued 2017-09
dc.description.abstract Microorganisms able to synthesize rhodopsins have the capacity to translocate ions through their membranes, using solar energy to generate a proton motive force. Rhodopsins are the most abundant phototrophic proteins in oceanic surface waters and are key constituents in marine bacterial ecology. However, it remains unclear how rhodopsins are used in most microorganisms. Despite their abundance in marine and fresh-water systems, the presence of functional rhodopsin systems in edaphic habitats has never been reported. Here, we show the presence of several new putative H+, Na+ and Cl+ pumping rhodopsins identified by metagenomic analysis of Antarctic desert hypolithic communities. Reconstruction of two Proteobacteria genomes harboring xanthorhodopsin-like proteins and one Bacteroidetes genome with a Na-pumping-like rhodopsin indicated that these bacteria were aerobic heterotrophs possessing the apparent capacity for the functional expression of rhodopsins. The existence of these protein systems in hypolithic bacteria expands the known role of rhodopsins to include terrestrial environments and suggests a possible predominant function as heterotrophic energy supply proteins, a feasible microbial adaptation to the harsh conditions prevalent in Antarctic edaphic systems. en_ZA
dc.description.department Genetics en_ZA
dc.description.embargo 2018-09-30
dc.description.librarian hj2017 en_ZA
dc.description.sponsorship The South African National Research Foundation SANAP [93074], Blue Skies [81693], the University of Pretoria Genomics Research Institute, the South African National Research Foundation and Claude Leon Foundation. en_ZA
dc.description.uri http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920 en_ZA
dc.identifier.citation Guerrero, L.D., Vikram, S., Makhalanyane, T.P. & Cowan, D.A. 2017, 'Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems', Environmental Microbiology, vol. 19, no. 9, pp. 3755-3767. en_ZA
dc.identifier.issn 1462-2912 (print)
dc.identifier.issn 1462-2920 (online)
dc.identifier.other 10.1111/1462-2920.13877
dc.identifier.uri http://hdl.handle.net/2263/62813
dc.language.iso en en_ZA
dc.publisher Wiley en_ZA
dc.rights © 2017 Society for Applied Microbiology and John Wiley & Sons Ltd. This is the pre-peer reviewed version of the following article : 'Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems', Environmental Microbiology, vol. 19, no. 9, pp. 3755-3767, 2017, doi : 10.1111/1462-2920.13877. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1462-2920. en_ZA
dc.subject Antarctic Dry Valley en_ZA
dc.subject Edaphic systems en_ZA
dc.subject Rhodopsins en_ZA
dc.subject Microorganisms en_ZA
dc.subject Antarctic desert hypolithic communities en_ZA
dc.subject Antarctic edaphic systems en_ZA
dc.title Evidence of microbial rhodopsins in Antarctic Dry Valley edaphic systems en_ZA
dc.type Postprint Article en_ZA


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