Consequences of endogenous and exogenous WNT signaling for development of the preimplantation bovine embryo

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dc.contributor.author Tribulo, Paula
dc.contributor.author Da Silva Leao, Beatriz Caetano
dc.contributor.author Lehloenya, Khoboso C.
dc.contributor.author Mingoti, Gisele Zoccal
dc.contributor.author Hansen, Peter J.
dc.date.accessioned 2017-09-12T09:17:26Z
dc.date.available 2017-09-12T09:17:26Z
dc.date.issued 2017-06
dc.description Supplemental File S1. Information on antibodies used. en_ZA
dc.description.abstract The specific role of WNT signaling during preimplantation development remains unclear. Here, we evaluated consequences of activation and inhibition of β-catenin (CTNNB1)-dependent and -independent WNT signaling in the bovine preimplantation embryo. Activation of CTNNB1- mediated WNT signaling by the agonist 2-amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3- methoxyphenyl)pyrimidine (AMBMP) and a glycogen synthase kinase 3 inhibitor reduced development to the blastocyst stage. Moreover, the antagonist of WNT signaling, dickkopf-related protein 1 (DKK1), alleviated the negative effect of AMBMP on development via reduction of CTNNB1. Based on labeling for phospho c-Jun N-terminal kinase, there was no evidence that DKK1 activated the planar cell polarity (PCP) pathway. Inhibition of secretion of endogenous WNTs did not affect development but increased number of cells in the inner cell mass (ICM). In contrast, DKK1 did not affect number of ICM or trophectoderm (TE) cells, suggesting that embryo-derived WNTs regulate ICM proliferation through a mechanism independent of CTNNB1. In addition, DKK1 did not affect the number of cells positive for the transcription factor yes-associated protein 1 (YAP1) involved in TE formation. In fact, DKK1 decreased YAP1. In contrast, exposure of embryos to WNT family member 7A (WNT7A) improved blastocyst development, inhibited the PCP pathway, and did not affect amounts of CTNNB1. Results indicate that embryo-derived WNTs are dispensable for blastocyst formation but participate in regulation of ICM proliferation, likely through a mechanism independent of CTNNB1. The response to AMBMP and WNT7A leads to the hypothesis that maternally derived WNTs can play a positive or negative role in regulation of preimplantation development. en_ZA
dc.description.department Animal and Wildlife Sciences en_ZA
dc.description.librarian am2017 en_ZA
dc.description.sponsorship The L.E. "Red" Larson Endowment, Agriculture and Food Research Initiative Competitive Grant no. 2011-67015-30688 from the United States Dept. of Agriculture National Institute of Food and Agriculture, and National Institutes of Health Grant R03 HD080855. en_ZA
dc.description.uri http://www.biolreprod.org en_ZA
dc.identifier.citation Tribulo, P., Leao, B.C.D., Lehloenya, K.C., Mingoti, G.Z. & Hansen, P.J. 2017, 'Consequences of endogenous and exogenous WNT signaling for development of the preimplantation bovine embryo', Biology of Reproduction, vol. 96, no. 6, pp. 1129-1141. en_ZA
dc.identifier.issn 0006-3363 (prrint)
dc.identifier.issn 1529-7268 (online)
dc.identifier.other 10.1093/biolre/iox048
dc.identifier.uri http://hdl.handle.net/2263/62238
dc.language.iso en en_ZA
dc.publisher Society for the Study of Reproduction en_ZA
dc.rights © The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/). en_ZA
dc.subject Embryo development en_ZA
dc.subject Preimplantation development en_ZA
dc.subject WNT en_ZA
dc.subject Dickkopf-related protein 1 (DKK1) en_ZA
dc.subject Planar cell polarity (PCP) en_ZA
dc.subject WNT family member 7A (WNT7A) en_ZA
dc.subject Mouse blastocyst en_ZA
dc.subject Human endometrium en_ZA
dc.subject Predicts outcomes en_ZA
dc.subject Gene expression en_ZA
dc.subject Trophectoderm lineage en_ZA
dc.subject Stem cells en_ZA
dc.subject Inner cell mass en_ZA
dc.subject Single blastocyst transfer en_ZA
dc.subject Colony stimulating factor 2 en_ZA
dc.title Consequences of endogenous and exogenous WNT signaling for development of the preimplantation bovine embryo en_ZA
dc.type Article en_ZA


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