Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions

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dc.contributor.author Koehler, Susanne M.
dc.contributor.author Buyuk, Fatih
dc.contributor.author Celebi, Ozgur
dc.contributor.author Demiraslan, Hayati
dc.contributor.author Doganay, Mehmet
dc.contributor.author Sahin, Mitat
dc.contributor.author Moehring, Jens
dc.contributor.author Ndumnego, Okechukwu Chinazo
dc.contributor.author Otlu, Salih
dc.contributor.author Van Heerden, Henriette
dc.contributor.author Beyer, Wolfgang
dc.date.accessioned 2017-08-25T09:03:18Z
dc.date.available 2017-08-25T09:03:18Z
dc.date.issued 2017-07-12
dc.description Additional file 1: Supporting tables. Table S1. Anti-rPA83 IgG titers. Table S2. TNA titers. Table S3. Anti-rBclA IgG titers. Table S4. Anti-FIS IgG titers. en_ZA
dc.description.abstract BACKGROUND : Bacillus (B.) anthracis, the causal agent of anthrax, is effectively controlled by the Sterne live spore vaccine (34F2) in animals. However, live spore vaccines are not suitable for simultaneous vaccination and antibiotic treatment of animals being at risk of infection in an outbreak situation. Non-living vaccines could close this gap. RESULTS : In this study a combination of recombinant protective antigen and recombinant Bacillus collagen-like antigen (rBclA) with or without formalin inactivated spores (FIS), targeted at raising an immune response against both the toxins and the spore of B. anthracis, was tested for immunogenicity and protectiveness in goats. Two groups of goats received from local farmers of the Kars region of Turkey were immunized thrice in three weeks intervals and challenged together with non-vaccinated controls with virulent B. anthracis, four weeks after last immunization. In spite of low or none measurable toxin neutralizing antibodies and a surprisingly low immune response to the rBclA, 80% of the goats receiving the complete vaccine were protected against a lethal challenge. Moreover, the course of antibody responses indicates that a two-step vaccination schedule could be sufficient for protection. CONCLUSION : The combination of recombinant protein antigens and FIS induces a protective immune response in goats. The non-living nature of this vaccine would allow for a concomitant antibiotic treatment and vaccination procedure. Further studies should clarify how this vaccine candidate performs in a post infection scenario controlled by antibiotics. en_ZA
dc.description.department Veterinary Tropical Diseases en_ZA
dc.description.librarian am2017 en_ZA
dc.description.sponsorship Deutsche Forschungsgemeinschaft (BE 2157/4–1) en_ZA
dc.description.uri http://www.biomedcentral.com/bmcvetres en_ZA
dc.identifier.citation Koehler, S.M., Buyuk, F., Celebi, O., Demiraslan, H., Doganay, M., Sahin, M., Moehring, J., Ndumnego, O.C., Otlu, S., Van Heerden, H. & Beyer, W. 2017, 'Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions', BMC Veterinary Research, vol. 13, art. no. 220, pp. 1-11. en_ZA
dc.identifier.issn 1746-6148 (online)
dc.identifier.other 10.1186/s12917-017-1140-2
dc.identifier.uri http://hdl.handle.net/2263/61803
dc.language.iso en en_ZA
dc.publisher BioMed Central en_ZA
dc.rights © The Author(s). 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/). en_ZA
dc.subject Bacillus anthracis en_ZA
dc.subject Anthrax en_ZA
dc.subject Vaccination en_ZA
dc.subject Animal vaccine en_ZA
dc.subject Exosporium en_ZA
dc.subject Immunization en_ZA
dc.subject Mice en_ZA
dc.subject Vaccines en_ZA
dc.subject DNA vaccination en_ZA
dc.subject Antigen en_ZA
dc.subject Suboptimal amounts en_ZA
dc.subject Comparative efficacy en_ZA
dc.subject Guinea pigs en_ZA
dc.title Protection of farm goats by combinations of recombinant peptides and formalin inactivated spores from a lethal Bacillus anthracis challenge under field conditions en_ZA
dc.type Article en_ZA


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