Figure S1 Optimization of plant-based expression of recombinant
AHSV-5 structural proteins.
Figure S2 Increased formation of AHSV-5 VLPs incorporating a
mutated version of VP7.
Figure S3 Purification of AHSV-5 VLPs.
Figure S4 Mass spectrometry analysis of the 4 protein bands
recovered from SDS-PAGE separation of density gradient fractions
from leaves co-infiltrated with Agrobacterium AGL1 pEAQ
recombinants for co-expression of AHSV capsid proteins VP2,
VP3, VP5 and VP7mu.
Figure S5 Quantification of AHSV-5 VLPs by gel densitometry.
Table S1 Virus neutralizing antibody titres of serum samples from
vaccinated and control guinea pigs V1 and C1.