Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing

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dc.contributor.author Helbing, Sophie
dc.contributor.author Lattorff, H. Michael G.
dc.contributor.author Moritz, Robin F.A.
dc.contributor.author Buttstedt, Anja
dc.date.accessioned 2017-07-31T09:15:10Z
dc.date.available 2017-07-31T09:15:10Z
dc.date.issued 2017
dc.description.abstract The western honeybee, Apis mellifera is a prominent model organism in the field of sociogenomics and a recent upgrade substantially improved annotations of the reference genome. Nevertheless, genome assemblies based on short-sequencing reads suffer from problems in regions comprising e.g. multi-copy genes. We used single-molecule nanopore-based sequencing with extensive read-lengths to reconstruct the organization of the major royal jelly protein (mrjp) region in three species of the genus Apis. Long-amplicon sequencing provides evidence for lineage-specific evolutionary fates of Apis mrjps. Whereas the most basal species, A. florea, seems to encode ten mrjps, different patterns of gene loss and retention were observed for A. mellifera and A. dorsata. Furthermore, we show that a previously reported pseudogene in A. mellifera, mrjp2-like, is an assembly artefact arising from short read sequencing. en_ZA
dc.description.department Zoology and Entomology en_ZA
dc.description.librarian am2017 en_ZA
dc.description.sponsorship The German Research Foundation (Deutsche Forschungsgemeinschaft - DFG, Grant MO 373/32-1 to RFAM). en_ZA
dc.description.uri http://dnaresearch.oxfordjournals.org en_ZA
dc.identifier.citation Helbing, S, Lattorff, HMG, Moritz, RFA & Buttstedt, A 2017, 'Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing', DNA Research, vol. 24, no. 3, pp. 279-287. en_ZA
dc.identifier.issn 1340-2838 (print)
dc.identifier.issn 1756-1663 (online)
dc.identifier.other 10.1093/dnares/dsw064
dc.identifier.uri http://hdl.handle.net/2263/61527
dc.language.iso en en_ZA
dc.publisher Oxford University Press en_ZA
dc.rights © The Author 2017. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/). en_ZA
dc.subject MinIONTM en_ZA
dc.subject Gene duplication en_ZA
dc.subject Apis mellifera en_ZA
dc.subject Apis florea en_ZA
dc.subject Apis dorsata en_ZA
dc.subject Major royal jelly protein (MRJP) en_ZA
dc.title Comparative analyses of the major royal jelly protein gene cluster in three Apis species with long amplicon sequencing en_ZA
dc.type Article en_ZA


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