dc.contributor.author |
Seo, Wonhyo
|
|
dc.contributor.author |
Prehaud, Christophe
|
|
dc.contributor.author |
Khan, Zakir
|
|
dc.contributor.author |
Sabeta, Claude Taurai
|
|
dc.contributor.author |
Lafon, Monique
|
|
dc.date.accessioned |
2017-07-25T06:03:30Z |
|
dc.date.issued |
2017-09 |
|
dc.description.abstract |
In the field of live viral vaccines production, there is an unmet need for in vitro tests complying a 3R approach (Refine, Replace and Reduce the use of animal experimentation) to replace the post-licensing safety tests currently assayed in animals. Here, we performed a pilot study evaluating whether virulence of rabies virus, RABV, can be forecast by an in vitro test of neurite outgrowth. The rationale to use neurite outgrowth as a read-out for this test is based on the salient property of the cytoplasmic domain of the G-protein (Cyto-G) of virulent RABV strains – not of attenuated RABV strains – to stimulate neurite outgrowth in vitro. We observed that neurite elongation triggered by the Cyto-Gs encoded by different RABV field isolates correlate with the distinct virulence scores obtained in a mouse model of experimental rabies. Our results cast the idea that it could be feasible to predict RABV virulence by testing the in vitro property of a RABV strain to promote neurite outgrowth without the use of animal experimentation. |
en_ZA |
dc.description.department |
Veterinary Tropical Diseases |
en_ZA |
dc.description.embargo |
2018-09-30 |
|
dc.description.librarian |
hj2017 |
en_ZA |
dc.description.sponsorship |
Institut Pasteur, Paris |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/micinf |
en_ZA |
dc.identifier.citation |
Seo, W., Prehaud, C., | Khan, Z., Sabeta, C.T. & Lafon. M. 2017, 'Investigation of rabies virus glycoprotein carboxyl terminus as an in vitro predictive tool of neurovirulence. A 3R approach', Microbes and Infection, vol. 19, no. 9-10, pp. 476-484. |
en_ZA |
dc.identifier.issn |
1769-714X (online) |
|
dc.identifier.issn |
1286-4579 (print) |
|
dc.identifier.other |
10.1016/j.micinf.2017.05.006 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/61426 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2017 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Microbes and Infection. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Microbes and Infection, vol. 19, no. 9-10, pp. 476-484, 2017. doi : 10.1016/j.micinf.2017.05.006. |
en_ZA |
dc.subject |
3R |
en_ZA |
dc.subject |
Post-licensing safety test |
en_ZA |
dc.subject |
Live viral vaccine |
en_ZA |
dc.subject |
Neurite outgrowth |
en_ZA |
dc.subject |
PDZ |
en_ZA |
dc.subject |
Rabies virus (RABV) |
en_ZA |
dc.title |
Investigation of rabies virus glycoprotein carboxyl terminus as an in vitro predictive tool of neurovirulence. A 3R approach |
en_ZA |
dc.type |
Postprint Article |
en_ZA |