The role of cell apoptosis on in vitroproduced beef cattle embryos

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dc.contributor.advisor Lehloenya, Khoboso C. en
dc.contributor.coadvisor Marle-Koster, Este en
dc.contributor.coadvisor Nedambale, T.L. en
dc.contributor.postgraduate Nkadimeng, Marble en
dc.date.accessioned 2017-06-05T12:12:04Z
dc.date.available 2017-06-05T12:12:04Z
dc.date.created 2017-04-05 en
dc.date.issued 2016 en
dc.description Dissertation (MSc (Agric))--University of Pretoria, 2016. en
dc.description.abstract The in vitro culture environment of cattle embryos can compromise the survival of developing embryos resulting in cell apoptosis. Detection of cell apoptosis is important for determining embryonic quality and reducing embryonic mortality in female animals prior to transfer. In this study the role of cell apoptosis on in vitro embryos was studied, with the focus on oocyte maturation and embryo production, caspase-3 activity and DNA fragmentation. Cow ovaries were collected from local abattoir and a total of 900 COCs were retrieved per week for the study over five-month period (six replicate/per experiments). COCs were randomly allocated to four incubation temperatures (39, 41, 42 and 43 ̊C) for polar body extrusion. Based on maturation results, two preferred temperatures (39 and 41 ̊C) were selected for maturation. Oocytes were subjected to normal subsequent embryonic conditions post maturation. Embryos produced from both maturation temperatures were then examined for embryonic development, caspase-3 activity and DNA fragmentation for evidence of apoptosis. No difference was (P<0.05) observed in embryonic development between oocytes matured at 39 ̊C and 41 ̊C respectively. Blastocysts produced at 39 ̊C showed significantly (P < 0.05) higher nuclei cells compared to those produced from 41 ̊C matured oocytes. A higher (P< 0.05) caspase-3 activity and DNA fragmentation were observed at (2-4 cell) and (≥ 8cell cell) embryos for 41 ̊C matured oocytes compared to 39 ̊C maturation group. A significantly higher caspase-3 activity and DNA fragmentation on blastocyst produced at 41 ̊C matured oocytes were also evident compared to those produced at 39 ̊C. Although embryos produced from oocytes matured at 41 ̊C showed similar developmental capacity to embryos from 39 ̊C, this study showed that a higher incidence of apoptosis can be expected in embryos produced from oocytes matured at a higher (41 ̊C) temperature than 39 ̊C. en_ZA
dc.description.availability Unrestricted en
dc.description.degree MSc (Agric) en
dc.description.department Animal and Wildlife Sciences en
dc.identifier.citation Nkadimeng, M 2016, The role of cell apoptosis on in vitroproduced beef cattle embryos, MSc (Agric) Dissertation, University of Pretoria, Pretoria, viewed yymmdd <http://hdl.handle.net/2263/60847> en
dc.identifier.other A2017 en
dc.identifier.uri http://hdl.handle.net/2263/60847
dc.language.iso en en
dc.publisher University of Pretoria en
dc.rights © 2017 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria. en
dc.subject UCTD en
dc.title The role of cell apoptosis on in vitroproduced beef cattle embryos en
dc.type Dissertation en


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