Ovine herpesvirus 2 (OvHV-2), is the causative agent of sheep-associated malignant
catarrhal fever (SA-MCF), a generally fatal disease of cattle and other captive wild ruminants.
Information on the OvHV-2 strains circulating in South Africa (SA) and other African
countries with regard to genetic structure and diversity, and pattern of distribution is not
available. This study aimed to characterize the OvHV-2 strains circulating in SA using
selected genes encoding glycoproteins and tegument proteins. To establish the genetic
diversity of OvHV-2 strains, four genes, Ov 7, Ov 8 ex2, ORF 27 and ORF 73 were selected
for analysis by PCR and DNA sequencing. Nucleotide and amino acid multiple sequence
analyses revealed two genotypes for ORF 27 and ORF 73, and three genotypes for Ov 7
and Ov 8 ex2, randomly distributed throughout the regions. Ov 7 and ORF 27 nucleotide
sequence analysis revealed variations that distinguished SA genotypes from those of reference
OvHV-2 strains. Epitope mapping analysis showed that mutations identified from
the investigated genes are not likely to affect the functions of the gene products, particularly
those responsible for antibody binding activities associated with B-cell epitopes.
Knowledge of the extent of genetic diversity existing among OvHV-2 strains has provided
an understanding on the distribution patterns of OvHV-2 strains or genotypes across the
regions of South Africa. This can facilitate the management of SA-MCF in SA, in terms of
introduction of control measures or safe practices to monitor and control OvHV-2 infection.
The products encoded by the Ov 7, Ov 8 ex2 and ORF 27 genes are recommended for
evaluation of their coded proteins as possible antigens in the development of an OvHV-2
specific serodiagnostic assay.
S1 Table. Average sequence identities determined for the Ov 7 nucleotide and amino
sequences obtained between South African Ov 7 sequences compared to reference
S2 Table. Average sequence identities determined for the Ov 8 ex2 nucleotide (Figure A)
and derived amino acid (Figure B) sequences obtained between South African OvHV-2
strains compared to reference strains.
S3 Table. Average sequence identities determined for the ORF 27 nucleotide and derived
amino acid sequences obtained between South African OvHV-2 strains compared to reference
S4 Table. Average sequence identities for the ORF 73 nucleotide and derived amino acid
sequences obtained between South African OvHV-2 strains compared to reference strains.