Mitochondrial transfer : implications for assisted reproductive technologies

Show simple item record Reznichenko, A.S. Huyser, Carin Pepper, Michael Sean 2017-05-31T05:43:23Z 2017-05-31T05:43:23Z 2016-12
dc.description.abstract The use of mitochondrial transfer as a clinic procedure is drawing closer to reality. Here we provide a detailed overview of mitochondrial transfer techniques – both established and recent – including pronuclear, spindle, ooplasmic and blastomere transfer. Reasons as to why some techniques are more suitable for the prevention of mitochondrial DNA disease than others, as well as the advantages and disadvantages of each methodology, are discussed. The possible clinical introduction of these techniques has raised concerns about the adverse effects they may have on resultant embryos and offspring. Success rates of each technique, embryo viability and developmental consequences post mitochondrial transfer are addressed through analysis of evidence obtained from both animal and human studies. Counterarguments against potential mitochondrial-nuclear genome incompatibility are also provided. Additional clinical applications ofmitochondrial transfer techniques are discussed. These include the rescue or enhancement of fertility in women of advanced maternal age or those suffering from diabetes. An alternative to using mitochondrialDNA transfer for germline therapies is the therapeutic use of somatic cell nuclear transfer for the generation of personalised stem cells. Although ethically challenging, this method could offer patients already suffering from mitochondrial DNA diseases a novel treatment option. en_ZA
dc.description.department Immunology en_ZA
dc.description.department Obstetrics and Gynaecology en_ZA
dc.description.librarian am2017 en_ZA
dc.description.sponsorship This work was supported by grants from the South African Medical Research Council Flagship Awards Project SAMRC-RFA-UFSP-01-2013/ STEM CELLS, the SAMRC Extramural Unit for Stem Cell Research and Therapy and the Institute for Cellular and Molecular Medicine of the University of Pretoria. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Reznichenko, AS, Huyser, C & Pepper, MS 2016, 'Mitochondrial transfer : implications for assisted reproductive technologies', Applied and Translational Genomics, vol. 11, pp. 40-47. en_ZA
dc.identifier.issn 2212-0661
dc.identifier.other 10.1016/j.atg.2016.10.001
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2016 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license. en_ZA
dc.subject Mitochondrial transfer en_ZA
dc.subject Clinic procedure en_ZA
dc.subject DNA en_ZA
dc.subject Therapies en_ZA
dc.title Mitochondrial transfer : implications for assisted reproductive technologies en_ZA
dc.type Article en_ZA

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