The production of immunity to Cl. chauvoei

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Authors

Mason, J.H.
Scheuber, J.R.

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Pretoria : The Government Printer

Abstract

1. Potent Cl. chauvoei vaccine (anaculture) may be made in horse-flesh infusion broth plus 1.0 per cent. peptone, 0.85 per cent. salt and 2-5 per cent. of serum and/or the haemolysed red cells of the horse, ox and sheep (meat particles one-third by volume). 2. Tryptic digests of horse muscle media produce satisfactory vaccines. 3. Boiled, dense suspensions of Cl. chauvoei injected subcutaneously into sheep produce a high degree of protection to culture injected intramuscularly. 4. The medium and method finally decided on as a routine measure was: ox-liver infusion broth (liver particles one-third by volume) plus 1.0 per cent. peptone, 0.85 per cent. salt and 1 per cent. glucose added after sterilization, pH 7.8, 36 hours’ incubation, and 0.5 per cent. formolization for three days at 37°C.

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Keywords

Veterinary medicine

Sustainable Development Goals

Citation

Mason, JH & Scheuber, JR 1936, 'The production of immunity to Cl. chauvoei’, Onderstepoort Journal of Veterinary Science and Animal Industry, vol. 7, no. 1, pp. 143-165.