dc.contributor.author |
Haig, D.A.
|
|
dc.contributor.editor |
Du Toit, P.J. |
|
dc.date.accessioned |
2017-04-06T13:41:11Z |
|
dc.date.available |
2017-04-06T13:41:11Z |
|
dc.date.created |
2017 |
|
dc.date.issued |
1948 |
|
dc.description |
The articles have been scanned in colour with a HP Scanjet 5590; 300dpi.
Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. |
en_ZA |
dc.description.abstract |
Green's distemper vaccine virus has been propagated 30 generations in developing hen eggs.
Injections were made onto the chorio-allantoic membranes of eggs that had received a preliminary incubation of 8 days. Re-incubation was done at 35°C., and passage of the membranes was made at approximately 4 day intervals.
The egg-adapted strain produced markedly oedematous changes with some necrosis in the membranes, but only occasionally killed the embryo. The titre of infected chorio-allantoic membranes, measured by ferret injection was between 10⁻³ and 10⁻⁵. That of the embryos and extra-enbryonic fluids of the same eggs was 10⁻³.
The reactions produced by the egg-adapted strain when injected into
ferrets were similar to those produced by the parent strain.
Five c.c. of anti-distemper serum neutralized the egg-cultured virus.
Thirteen dogs were inoculated with suspensions of chorio-allantoic membranes.
In nine there was no reaction. In one there was a slight reaction,
but in three the reactions were very severe and one died after showing
typical distemper symptoms.
The egg-adapted virus did not agglutinate chicken or guinea pig red
cells and failed to infect mice when instilled intra-nasally. |
en_ZA |
dc.identifier.citation |
Haig, DA 1948, 'Preliminary note on the cultivation of Green's distemperoid virus in fertile hen eggs’, Onderstepoort Journal of Veterinary Science and Animal Industry, vol. 23, nos. 1 & 2, pp. 149-155. |
en_ZA |
dc.identifier.issn |
0330-2465 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/59693 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Pretoria : The Government Printer |
en_ZA |
dc.rights |
© 1948 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2017 University of Pretoria. Dept. of Library Services (digital). |
en_ZA |
dc.subject |
Veterinary medicine |
en_ZA |
dc.subject.lcsh |
Veterinary medicine -- South Africa |
|
dc.title |
Preliminary note on the cultivation of Green's distemperoid virus in fertile hen eggs |
en_ZA |
dc.type |
Article |
en_ZA |