Preliminary note on the cultivation of Green's distemperoid virus in fertile hen eggs

Show simple item record Haig, D.A.
dc.contributor.editor Du Toit, P.J. 2017-04-06T13:41:11Z 2017-04-06T13:41:11Z 2017 1948
dc.description The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format. en_ZA
dc.description.abstract Green's distemper vaccine virus has been propagated 30 generations in developing hen eggs. Injections were made onto the chorio-allantoic membranes of eggs that had received a preliminary incubation of 8 days. Re-incubation was done at 35°C., and passage of the membranes was made at approximately 4 day intervals. The egg-adapted strain produced markedly oedematous changes with some necrosis in the membranes, but only occasionally killed the embryo. The titre of infected chorio-allantoic membranes, measured by ferret injection was between 10⁻³ and 10⁻⁵. That of the embryos and extra-enbryonic fluids of the same eggs was 10⁻³. The reactions produced by the egg-adapted strain when injected into ferrets were similar to those produced by the parent strain. Five c.c. of anti-distemper serum neutralized the egg-cultured virus. Thirteen dogs were inoculated with suspensions of chorio-allantoic membranes. In nine there was no reaction. In one there was a slight reaction, but in three the reactions were very severe and one died after showing typical distemper symptoms. The egg-adapted virus did not agglutinate chicken or guinea pig red cells and failed to infect mice when instilled intra-nasally. en_ZA
dc.identifier.citation Haig, DA 1948, 'Preliminary note on the cultivation of Green's distemperoid virus in fertile hen eggs’, Onderstepoort Journal of Veterinary Science and Animal Industry, vol. 23, nos. 1 & 2, pp. 149-155. en_ZA
dc.identifier.issn 0330-2465
dc.language.iso en en_ZA
dc.publisher Pretoria : The Government Printer en_ZA
dc.rights © 1948 ARC - Onderstepoort and Faculty of Veterinary Science, University of Pretoria (original). © 2017 University of Pretoria. Dept. of Library Services (digital). en_ZA
dc.subject Veterinary medicine en_ZA
dc.subject.lcsh Veterinary medicine -- South Africa
dc.title Preliminary note on the cultivation of Green's distemperoid virus in fertile hen eggs en_ZA
dc.type Article en_ZA

Files in this item

This item appears in the following Collection(s)

Show simple item record