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dc.contributor.author | Danaviah, Sivapragashini![]() |
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dc.contributor.author | De Oliveira, Tulio![]() |
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dc.contributor.author | Gordon, Michelle![]() |
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dc.contributor.author | Govender, Shunmugam![]() |
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dc.contributor.author | Chelule, Paul![]() |
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dc.contributor.author | Pillay, Sureshnee![]() |
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dc.contributor.author | Naicker, Thajasvarie![]() |
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dc.contributor.author | Cassol, Sharon![]() |
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dc.contributor.author | Ndung'u, Thumbi![]() |
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dc.date.accessioned | 2017-03-23T08:17:06Z | |
dc.date.issued | 2016-03 | |
dc.description.abstract | Extrapulmonary tuberculosis (TB) is a significant public health challenge in South Africa and worldwide, largely fuelled by the HIV epidemic. In spinal TB, Mycobacteria infect the spinal column without dissemination to the spinal cord. The immune microenvironment, target cell characteristics, and other evolutionary forces within granulomas during HIV/TB coinfection are poorly characterized. We investigated whether spinal TB granulomas represent a sequestered anatomical site where independent HIV evolution occurs, and assessed the role of macrophages as a target cell for both HIV and mycobacteria. RNA was extracted from plasma and granulomatous tissue from six antiretroviral-naive HIV-1/spinal TB-coinfected patients, RT-PCR amplified, and the C2-V5 env segment was cloned and sequenced. Analysis of genetic diversity, phylogeny and coalescence patterns was performed on clonal sequences. To investigate their role in HIV sequestration, macrophages and the HIV-1 p24 protein were immune localized and ultrastructural features were studied. Intercompartment diversity measurements and phylogenetic reconstruction revealed anatomically distinct monophyletic HIV-1 clusters in four of six patients. Genotypic CCR5-tropic variants were predominant (98.9%) with conservation of putative N-linked glycosylation sites in both compartments. CD68+ reactivity was associated with higher tissue viral load (r = 1.0; p < 0.01) but not greater intrapatient diversity (r = 0.60; p > 0.05). Ultrastructural imaging revealed the presence of bacterial and virus-like particles within membrane-bound intracellular compartments of macrophages. Spinal tuberculosis granulomas may form anatomically discreet sites of divergent viral evolution. Macrophages in these granulomas harbored both pathogens, suggesting that they may facilitate the process of viral sequestration within this compartment. | en_ZA |
dc.description.department | Immunology | en_ZA |
dc.description.embargo | 2017-03-31 | |
dc.description.librarian | hb2017 | en_ZA |
dc.description.sponsorship | This work was funded by a Wellcome Trust Grant. T. Ndung'u is funded through the South African DST/NRF Research Chair in Systems Biology of HIV/AIDS, the Victor Daitz Chair in HIV/TB Research, and an International Early Career Scientist Award from the Howard Hughes Medical Institute. | en_ZA |
dc.description.uri | http://online.liebertpub.com/aid | en_ZA |
dc.identifier.citation | Danaviah, S, De Oliveira, T, Gordon, M, Govender, S, Chelule, P, Pillay, S, Naicker, T, Cassol, S & Ndung'u, T 2016, 'Analysis of dominant HIV quasispecies suggests independent viral evolution within spinal granulomas coinfected with mycobacterium tuberculosis and HIV-1 Subtype C', AIDS Research and Human Retroviruses, vol. 32, no. 3, pp. 262-270. | en_ZA |
dc.identifier.issn | 0889-2229 (print) | |
dc.identifier.issn | 1931-8405 (online) | |
dc.identifier.other | 10.1089/aid.2015.0189 | |
dc.identifier.uri | http://hdl.handle.net/2263/59505 | |
dc.language.iso | en | en_ZA |
dc.publisher | Mary Ann Liebert | en_ZA |
dc.rights | © 2016 Mary Ann Liebert, Inc. All rights reserved. | en_ZA |
dc.subject | Extrapulmonary tuberculosis (TB) | en_ZA |
dc.subject | Mycobacteria | en_ZA |
dc.subject | Spinal tuberculosis | en_ZA |
dc.subject | Human immunodeficiency virus (HIV) | en_ZA |
dc.subject | Mycobacterium tuberculosis (MTB) | en_ZA |
dc.title | Analysis of dominant HIV quasispecies suggests independent viral evolution within spinal granulomas coinfected with mycobacterium tuberculosis and HIV-1 Subtype C | en_ZA |
dc.type | Postprint Article | en_ZA |