Proteomic and metabolomic analysis reveals rapid and extensive nicotine detoxification ability in honey bee larvae

Show simple item record Du Rand, Esther Elizabeth Human, Hannelie Smit, Salome Beukes, Mervyn Apostolides, Zeno Nicolson, Sue W. Pirk, Christian Walter Werner 2017-03-09T13:24:39Z 2017-03
dc.description.abstract Despite potential links between pesticides and bee declines, toxicology information on honey bee larvae (Apis mellifera) is scarce and detoxification mechanisms in this development stage are virtually unknown. Larvae are exposed to natural and synthetic toxins present in pollen and nectar through consumption of brood food. Due to the characteristic intensive brood care displayed by honey bees, which includes progressive feeding throughout larval development, it is generally assumed that larvae rely on adults to detoxify for them and exhibit a diminished detoxification ability. We found the opposite. We examined the proteomic and metabolomic responses of in vitro reared larvae fed nicotine (an alkaloid found in nectar and pollen) to understand how larvae cope on a metabolic level with dietary toxins. Larvae were able to effectively detoxify nicotine through an inducible detoxification mechanism. A coordinated stress response complemented the detoxification processes, and we detected significant enrichment of proteins functioning in energy and carbohydrate metabolism, as well as in development pathways, suggesting that nicotine may promote larval growth. Further exploration of the metabolic fate of nicotine using targeted mass spectrometry analysis demonstrated that, as in adult bees, formation of 4-hydroxy-4-(3-pyridyl) butanoic acid, the result of 2’C-oxidation of nicotine, is quantitatively the most significant pathway of nicotine metabolism. We provide conclusive evidence that larvae are capable of effectively catabolising a dietary toxin, suggesting that increased larval sensitivity to specific toxins is not due to diminished detoxification abilities. These findings broaden the current understanding of detoxification biochemistry at different organizational levels in the colony, bringing us closer to understanding the capacity of the colony as a superorganism to tolerate and resist toxic compounds, including pesticides, in the environment. en_ZA
dc.description.department Biochemistry en_ZA
dc.description.department Zoology and Entomology en_ZA
dc.description.embargo 2018-03-31
dc.description.librarian hb2017 en_ZA
dc.description.sponsorship This work was funded jointly by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC), Natural Environment Research Council (NERC), the Wellcome Trust, Defra, and the Scottish Government under the Insect Pollinators Initiative (BB/ I000968/1). We also acknowledge the support of the National Research Foundation of South Africa (73671) and the University of Pretoria. en_ZA
dc.description.uri en_ZA
dc.identifier.citation Du Rand, EE, Human, H, Smit, S, Beukes, M, Apostolides, Z, Nicolson, SW & Pirk, CWW 2017, 'Proteomic and metabolomic analysis reveals rapid and extensive nicotine detoxification ability in honey bee larvae', Insect Biochemistry and Molecular Biology, vol. 82, pp. 41-51. en_ZA
dc.identifier.issn 0965-1748 (print)
dc.identifier.issn 1879-0240 (online)
dc.identifier.other 10.1016/j.ibmb.2017.01.011
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2017 Elsevier Ltd. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Insect Biochemistry and Molecular Biology . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Insect Biochemistry and Molecular Biology, vol. 82, pp. 41-51, 2017. doi : 10.1016/j.ibmb.2017.01.011. en_ZA
dc.subject Larvae en_ZA
dc.subject Nicotine en_ZA
dc.subject Detoxification en_ZA
dc.subject Energy metabolism en_ZA
dc.subject Honeybee (Apis mellifera) en_ZA
dc.title Proteomic and metabolomic analysis reveals rapid and extensive nicotine detoxification ability in honey bee larvae en_ZA
dc.type Postprint Article en_ZA

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