Cultivable microbiome of fresh white button mushrooms

Show simple item record Rossouw, Werner Korsten, Lise 2017-02-16T06:05:03Z 2017-02
dc.description.abstract Microbial dynamics on commercially grown white button mushrooms is of importance in terms of food safety assurance and quality control. The purpose of this study was to establish the microbial profile of fresh white button mushrooms, with the focus on potential presence of food-borne pathogens. The total microbial load was determined through standard viable counts. Presence and isolation of gram-negative bacteria including coagulase positive Staphylococci were performed using a selective enrichment approach. Dominant and presumptive organisms were confirmed using molecular methods. Total mushroom microbial counts ranged from 5.2 to 12.4 log cfu g-1, with the genus Pseudomonas being most frequently isolated (45.37% of all isolations). In total, 91 different microbial species were isolated and identified using Matrix assisted laser desorption ionisation time of flight mass spectrophotometry, PCR and sequencing. Considering current food safety guidelines in South Africa for ready-to-eat fresh produce, coliform counts exceeded the guidance specifications for fresh fruit and vegetables. Based on our research and similar studies, it is proposed that specifications for microbial loads on fresh, healthy mushrooms reflect a more natural microbiome at the pointof- harvest and point-of-sale. en_ZA
dc.description.department Plant Science en_ZA
dc.description.embargo 2018-02-28
dc.description.librarian hb2017 en_ZA
dc.description.sponsorship The National Research Foundation (NRF) and the Technology and Human Resources Industry Programme (THRIP), a partnership programme funded by the Department of Trade and Industry and managed by the NRF and the Department of Science and Technology-National Research Foundation funded Centre of Excellence in Food Security’s Food Safety Programme (Project 140701: Fresh Produce Safety). en_ZA
dc.description.uri en_ZA
dc.identifier.citation Rossouw, W & Korsten, L 2017, 'Cultivable microbiome of fresh white button mushrooms', Letters in Applied Microbiology, vol. 64, no. 2, pp. 164-170. en_ZA
dc.identifier.issn 0266-8254 (print)
dc.identifier.issn 1472-765X (online)
dc.identifier.other 10.1111/lam.12698
dc.language.iso en en_ZA
dc.publisher Wiley en_ZA
dc.rights © 2016 The Society for Applied Microbiology. This is the pre-peer reviewed version of the following article : Cultivable microbiome of fresh white button mushrooms, Letters in Applied Microbiology, vol. 64, no. 2, pp. 164-170. 2017. doi :10.1111/lam.12698. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1472-765X. en_ZA
dc.subject Food-borne pathogens en_ZA
dc.subject Agaricus bisporus microbiome en_ZA
dc.subject Cultivable microbiome of mushrooms en_ZA
dc.subject Button mushroom en_ZA
dc.title Cultivable microbiome of fresh white button mushrooms en_ZA
dc.type Postprint Article en_ZA

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