Diversity of free-living nitrogen fixing Streptomyces in soils of the badlands of South Dakota

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dc.contributor.author Dahal, Bibha
dc.contributor.author NandaKafle, Gitanjali
dc.contributor.author Perkins, Lora
dc.contributor.author Brözel, V.S. (Volker Siegfried), 1963-
dc.date.accessioned 2017-02-14T10:26:38Z
dc.date.issued 2017-01
dc.description.abstract Biological Nitrogen Fixation is critical for ecosystem productivity. Select members of Bacteria and Archaea express a nitrogenase enzyme complex that reduces atmospheric nitrogen to ammonia. Several nitrogen fixing bacteria form symbiotic associations with plants, but freeliving diazotrophs also contribute a substantial amount of nitrogen to ecosystems. The aim of this study was to isolate and characterize free-living diazotrophs in arid lands of South Dakota Badlands. Samples were obtained from sod tables and the surrounding base in spring and fall. Diazotrophs were isolated on solid nitrogen free medium (NFM) under hypoxic conditions, and their16S rRNA and nifH genes sequenced. nifH was also amplified directly from soil DNA extracts. The 16S rRNA gene data indicated a diversity of putative free-living diazotrophs across 4 phyla (Actinomycetes, Proteobacteria, Bacteroidetes, and Firmicutes), but ~50% of these clustered with Streptomyces. These Streptomyces isolates grew in liquid NFM in an ammoniadepleted environment. Only 5 of these yielded a nifH gene product using the PolF/PolR primer set. Four of these aligned with nifH of the cyanobacteria Scytonema and Nostoc, and the other one aligned with nifH of Bradyrhizobium. Six selected Streptomyces isolates, three of which were nifH positive by PCR, all indicated 15N2 incorporation, providing strong support of nitrogen fixation. All nifH amplicons from soil DNA extract resembled Cyanobacteria. This is the first known report of diazotrophic Streptomyces, other than the thermophilic, autotrophic S. thermoautotrophicus. nifH genes of these Streptomyces were related to those from Cyanobacteria. It is possible that the cyanobacteria-like nifH amplicons obtained from soil DNA were associated with Streptomyces. en_ZA
dc.description.department Microbiology and Plant Pathology en_ZA
dc.description.embargo 2018-01-31
dc.description.librarian hb2017 en_ZA
dc.description.sponsorship BD was supported by a fellowship from the South Dakota Agricultural Experiment Station. This work was supported by the South Dakota Agricultural Experiment Station. We acknowledge use of the SDSU-Functional Genomics Core Facility, supported by NSF/EPSCoR Grant No. 0091948, the South Dakota 2010 Drought Initiative, and the South Dakota Agricultural Experiment Station. en_ZA
dc.description.uri http://www.elsevier.com/locate/micres en_ZA
dc.identifier.citation Dahal, B, Nandakafle, G, Perkins, L & Brözel, VS 2017, 'Diversity of free-living nitrogen fixing Streptomyces in soils of the badlands of South Dakota', Microbiological Research, vol. 195, pp. 31-39. en_ZA
dc.identifier.issn 0944-5013 (print)
dc.identifier.issn 1618-0623(online)
dc.identifier.other 10.1016/j.micres.2016.11.004
dc.identifier.uri http://hdl.handle.net/2263/59030
dc.language.iso en en_ZA
dc.publisher Elsevier en_ZA
dc.rights © 2016 Elsevier GmbH. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in Microbiological Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in Microbiological Research, vol. 195, pp. 31-39, 2017. doi : 10.1016/j.micres.2016.11.004. en_ZA
dc.subject Diazotroph en_ZA
dc.subject Nitrogen fixing en_ZA
dc.subject Free-living en_ZA
dc.subject Badlands en_ZA
dc.subject Nitrogenase en_ZA
dc.subject Streptomyces en_ZA
dc.subject Nitrogen free medium (NFM) en_ZA
dc.title Diversity of free-living nitrogen fixing Streptomyces in soils of the badlands of South Dakota en_ZA
dc.type Postprint Article en_ZA


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