dc.contributor.author |
Van Wyk, Schalk J. P.
|
|
dc.contributor.author |
Boutigny, Anne-Laure
|
|
dc.contributor.author |
Coutinho, Teresa A.
|
|
dc.contributor.author |
Viljoen, Altus
|
|
dc.date.accessioned |
2017-01-11T05:10:53Z |
|
dc.date.available |
2017-01-11T05:10:53Z |
|
dc.date.issued |
2012-06 |
|
dc.description.abstract |
Pitch canker, caused by Fusarium circinatum, was first reported in a
forestry nursery in the Mpumalanga Province of South Africa in 1990,
and it has since spread to almost all forestry nurseries in the country,
where it causes significant economic losses. The aim of the current
study was to (i) identify sources of F. circinatum contamination in the
Karatara forestry nursery in the Western Cape Province and (ii) manage
the disease by implementing an oxidation reduction potential
(ORP)-based sanitation method using hydrogen peroxide. The irrigation
water, planting tray inserts and seeds were screened for fungal
contamination. Fusarium circinatum colonies were identified morphologically
and confirmed by polymerase chain reaction using speciesspecific
primers. Both the irrigation water and planting tray inserts served as sources of inoculum that introduced the pathogen into the
nursery. The irrigation water was amended with hydrogen peroxide at
an ORP level of 400 mV for an exposure time of 6 h because it was
observed that such a treatment effectively killed all F. circinatum
spores and was not phytotoxic to pine seedlings under laboratory
conditions. In addition, the contaminated planting tray inserts were
cleaned in water amended with hydrogen peroxide at an ORP value of
360 mV for 6 h, which was shown to efficiently eliminate all inoculum
from planting tray inserts. Since the introduction of the ORP-based
sanitation method at Karatara nursery, losses of pine seedlings were
reduced to insignificant levels, and field losses were minimized. |
en_ZA |
dc.description.department |
Microbiology and Plant Pathology |
en_ZA |
dc.description.librarian |
hb2016 |
en_ZA |
dc.description.uri |
http://apsjournals.apsnet.org/loi/pdis |
en_ZA |
dc.identifier.citation |
Van Wyk, SJP, Boutigny, A-L, Coutinho, TA & Viljoen, A 2012, 'Sanitation of a South African forestry nursery contaminated with Fusarium circinatum using hydrogen peroxide at specific oxidation reduction potentials', Plant Disease, vol. 96, no. 6, pp. 875-880. |
en_ZA |
dc.identifier.issn |
0191-2917 (print) |
|
dc.identifier.issn |
1943-7692 (online) |
|
dc.identifier.other |
10.1094/PDIS-05-11-0432 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/58475 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
American Phytopathological Society |
en_ZA |
dc.rights |
© 2012 The American Phytopathological Society. This is an open access article. |
en_ZA |
dc.subject |
Pitch canker |
en_ZA |
dc.subject |
Fusarium circinatum |
en_ZA |
dc.subject |
Hydrogen peroxide |
en_ZA |
dc.subject |
Contaminated planting |
en_ZA |
dc.title |
Sanitation of a South African forestry nursery contaminated with Fusarium circinatum using hydrogen peroxide at specific oxidation reduction potentials |
en_ZA |
dc.type |
Article |
en_ZA |