dc.contributor.author |
Abu Samra, Nada
|
|
dc.contributor.author |
Thompson, P.N. (Peter N.)
|
|
dc.contributor.author |
Jori, Ferran J.
|
|
dc.contributor.author |
Frean, J.
|
|
dc.contributor.author |
Poonsamy, B.
|
|
dc.contributor.author |
Du Plessis, D.
|
|
dc.contributor.author |
Mogoye, B.
|
|
dc.contributor.author |
Xiao, L.
|
|
dc.date.accessioned |
2016-12-12T06:39:31Z |
|
dc.date.available |
2016-12-12T06:39:31Z |
|
dc.date.issued |
2013-03 |
|
dc.description.abstract |
The diversity of Cryptosporidium at species, subtype family and subtype level in diarrhoeic
children was investigated in four provinces in South Africa. A total of 442 stool samples from
children <5 years of age were collected under a large rotavirus surveillance programme and
analysed by Ziehl–Neelsen acid-fast staining. Fifty-four (12.2%) were positive for
Cryptosporidium, of which 25 were genotyped by polymerase chain reaction (PCR)–
restriction fragment length polymorphism (RFLP) and DNA sequence analyses of the 18S
rRNA gene. The majority of genotyped specimens were identified as C. hominis (76%), and a
high genetic diversity was found with five different C. hominis subtype families (Ia, Ib, Id, Ie
and If). Cryptosporidium parvum was found in 20% of the isolates, and three subtype
families were identified (IIc, IIe and IIb), with subtype family IIc being the most common.
One specimen was identified as C. meleagridis of the subtype family IIId. These results are in
accordance with findings from other developing countries and report for the first time the
presence in South Africa of C. meleagridis, various subtypes of C. parvum and the subtype
family Ie of C. hominis. The results suggest that C. hominis and anthroponotic C. parvum
subtypes are the major cause of cryptosporidiosis in South Africa. Further molecular studies
are needed to better understand the epidemiology and public health importance of
Cryptosporidium in humans in South Africa. |
en_ZA |
dc.description.department |
Production Animal Studies |
en_ZA |
dc.description.librarian |
hb2016 |
en_ZA |
dc.description.sponsorship |
The Faculty of Veterinary Science, University of Pretoria, South Africa |
en_ZA |
dc.description.uri |
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1863-2378 |
en_ZA |
dc.identifier.citation |
Abu Samra, N, Thompson, PN, Jori, F, Frean, J, Poonsamy, B & Du Plessis, D 2013, 'Genetic characterization of Cryptosporidium spp. in diarrhoeic children from four provinces in South Africa', Zoonoses and Public Health, vol. 60, no. 2, pp. 154-159. |
en_ZA |
dc.identifier.issn |
1863-1959 (print) |
|
dc.identifier.issn |
1863-2378 (online) |
|
dc.identifier.other |
10.1111/j.1863-2378.2012.01507.x |
|
dc.identifier.uri |
http://hdl.handle.net/2263/58390 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Wiley |
en_ZA |
dc.rights |
© 2012 Blackwell Verlag GmbH. This is the pre-peer reviewed version of the following article : Genetic characterization of Cryptosporidium spp. in diarrhoeic children from four provinces in South Africa, Zoonoses and Public Health, vol. 60, no. 2, pp. 154-159, 2013. doi : 10.1111/j.1863-2378.2012.01507.x. The definite version is available at : http://onlinelibrary.wiley.comjournal/10.1111/(ISSN)1863-2378. |
en_ZA |
dc.subject |
Cryptosporidium |
en_ZA |
dc.subject |
Diarrhoeic children |
en_ZA |
dc.subject |
Public health |
en_ZA |
dc.subject |
South Africa (SA) |
en_ZA |
dc.subject.other |
Veterinary science articles SDG-03 |
en_ZA |
dc.subject.other |
SDG-03: Good health and well-being |
|
dc.title |
Genetic characterization of Cryptosporidium spp. in diarrhoeic children from four provinces in South Africa |
en_ZA |
dc.type |
Postprint Article |
en_ZA |