dc.contributor.author |
Yatsu, Ryohei
|
|
dc.contributor.author |
Katsu, Yoshinao
|
|
dc.contributor.author |
Kohno, Satomi
|
|
dc.contributor.author |
Mizutani, Takeshi
|
|
dc.contributor.author |
Ogino, Yukiko
|
|
dc.contributor.author |
Ohta, Yasuhiko
|
|
dc.contributor.author |
Myburgh, Jan G.
|
|
dc.contributor.author |
Van Wyk, Johannes H.
|
|
dc.contributor.author |
Guillette Jr., Louis J.
|
|
dc.contributor.author |
Miyagawa, Shinichi
|
|
dc.contributor.author |
Iguchi, Taisen
|
|
dc.date.accessioned |
2016-12-05T11:24:36Z |
|
dc.date.issued |
2016-11 |
|
dc.description.abstract |
Steroid hormones are a key regulator of reproductive biology in vertebrates, and are largely
regulated via nuclear receptor families. Estrogen signaling is regulated by two estrogen
receptor (ER) subtypes alpha and beta in the nucleus. In order to understand the role of
estrogen in vertebrates, these ER from various species have been isolated and were
functionally analyzed using luciferase reporter gene assays. Interestingly, species difference
in estrogen sensitivity has been noted in the past, and it was reported that snake ER displayed
highest estrogen sensitivity. Here, we isolated additional ER from three lizards: chameleon
(Bradypodion pumilum), skink (Plestiodon finitimus), and gecko (Gekko japonicus). We have
performed functional characterization of these ERs using reporter gene assay system, and
found high estrogen sensitivity in all three species. Furthermore, comparison with results 2
from other tetrapod ER revealed a seemingly uniform gradual pattern of ligand sensitivity
evolution. In silico 3D homology modeling of the ligand-binding domain revealed structural
variation at three sites, helix 2, and juncture between helices 8 and 9, and caudal region of
helix 10/11. Docking simulations indicated that predicted ligand-receptor interaction also correlated with the reporter assay results, and overall squamates displayed highest stabilized interactions. The assay system and homology modeling system provides tool for in-depth comparative analysis of estrogen function, and provides insight toward the evolution of ER among vertebrates. |
en_ZA |
dc.description.department |
Paraclinical Sciences |
en_ZA |
dc.description.embargo |
2017-11-30 |
|
dc.description.librarian |
hb2016 |
en_ZA |
dc.description.sponsorship |
This study was partially funded by the Grants-in-Aid for Scientific Research from the
Ministry of Education, Culture, Sports, Science and Technology of Japan (YO, SM, TI),
Japan Society for the Promotion of Science (RY), UK-Japan Research Collaboration Grants
from the Ministry of the Environment, Japan, and by the NIBB International Collaborative
Research Initiative (SM, TI). |
en_ZA |
dc.description.uri |
http://www.elsevier.com/locate/ygcen |
en_ZA |
dc.identifier.citation |
Yatsu, R, Katsu, Y, Kohno, S, Mizutani, T, Ogino, Y, Ohta, Y, Myburgh, J, Van Wyk, JH, Guillette, LJ, Miyagawa, S & Iguchi, T 2016, 'Characterization of evolutionary trend in squamate estrogen receptor sensitivity', General and Comparative Endocrinology, vol. 238, pp. 88-95 |
en_ZA |
dc.identifier.issn |
0016-6480 (print) |
|
dc.identifier.issn |
1095-6840 (online) |
|
dc.identifier.other |
10.1016/j.ygcen.2016.04.005 |
|
dc.identifier.uri |
http://hdl.handle.net/2263/58349 |
|
dc.language.iso |
en |
en_ZA |
dc.publisher |
Elsevier |
en_ZA |
dc.rights |
© 2016 Elsevier Inc. All rights reserved. Notice : this is the author’s version of a work that was accepted for publication in General and Comparative Endocrinology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. A definitive version was subsequently published in General and Comparative Endocrinology, vol. 238, pp. 88-95, 2016. doi : 10.1016/j.ygcen.2016.04.005. |
en_ZA |
dc.subject |
Squamates |
en_ZA |
dc.subject |
Estrogen sensitivity |
en_ZA |
dc.subject |
Evolution of estrogen receptor |
en_ZA |
dc.subject |
Estrogen receptor (ER) |
en_ZA |
dc.subject |
Vertebrates |
en_ZA |
dc.subject |
Steroid hormones |
en_ZA |
dc.subject |
Reproduction |
en_ZA |
dc.title |
Characterization of evolutionary trend in squamate estrogen receptor sensitivity |
en_ZA |
dc.type |
Postprint Article |
en_ZA |