S1 Fig. Test comb appearance in colony 2. Scans were performed at A) day 1, B) day 2, C) day
5, D) day 8, E) day 12 after feeding. Cell density patterns observed on each day are depicted by icons on the right side of each picture. Note 1) the increasing density and number of filled cells;
2) the changing shape of the area of nectar containing cells due to the relocation of cell content
after workers cleared cells for brood rearing (empty central area in D and E); such changes (1
and 2) also occurred in the other two colonies but with a lower frequency; 3) the dense areas of
cell content neighbouring empty cells.
S2 Fig. Sugar concentration (left y-axis) and filling level (right y-axis) over time in ten individual
cells per colony. Each row corresponds to a colony and shows a representative subsample
of filling and ripening dynamics. The first five cells of each line represent early provisioned
cells that contained solutions already at day 1 (some were relocated at a later stage); the following
5 cells represent eventually capped cells.
S1 Table. Results of Wilcoxon test comparing the filling and content concentration of early
provisioned and eventually capped cells at each scan day. Significant P—values (< 0.025)
after Bonferroni correction are indicated with .
S2 Table. Comparison of cell filling level and content concentration between consecutive
days in early provisioned and eventually capped cells. Significant P—values (< 0.05) from
the robust-ranked method (nparLD) are indicated with .
S3 Table. Results from the two-sample tests for equality of proportions between consecutive
days. The test was performed for each pattern category and for the three colonies separately.
Decrease or increase of proportions between the two days are indicated with < and >,
respectively. Significant P—values (< 0.05) are indicated with .
S4 Table. Results of the Poisson models (log-link in glm(), R) applied for pairwise comparisons
of total number of cells filled between consecutive days in each colony. Decreasing and
increasing values are indicated with < and >, respectively. Significant P-values (< 0.05) are
indicated with .
S5 Table. Wilcoxon-Mann-Whitney test comparing the amount of solution stored in the
three colonies between consecutive days.