Flow cytometric comparison of platelets from a whole blood and finger-prick sample : impact of 24 hours storage

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dc.contributor.author Swanepoel, Albe
dc.contributor.author Stander, Andre
dc.contributor.author Pretorius, Etheresia
dc.date.accessioned 2016-11-14T12:33:43Z
dc.date.available 2016-11-14T12:33:43Z
dc.date.issued 2013-07
dc.description.abstract In this study, we investigate the validity and laboratory utility of flow cytometry when analyzing platelet activation by studying CD41, CD42b, CD62P and CD63. We compare flow cytometry results from citrated whole blood and finger-prick samples directly after collection and also after storing both a finger-prick and whole blood sample for 24 hours. Citrated whole blood and finger-prick samples were taken from three healthy individuals on two occasions, and a total of 60 000 cells were analyzed for each of the 4 phycoerythrin-labelled monoclonal antibodies. Half of each sample was analyzed immediately after sampling while the other half was kept in the fridge at 6°C for 24 hours before analysis. No significant difference was found between the sampling methods or the period of time before analysis. Results therefore suggest that an appropriately prepared finger-prick sample can be used for platelet function analysis, and samples can be stored for 24 hours in the fridge at 6°C before analysis. en_ZA
dc.description.department Physiology en_ZA
dc.description.librarian hb2016 en_ZA
dc.description.uri http://www.maneyonline.com/loi/hem en_ZA
dc.identifier.citation Swanepoel, AC, Stander, A & Pretorius, E 2013, 'Flow cytometric comparison of platelets from a whole blood and finger-prick sample : impact of 24 hours storage', Hematology, vol. 18, no. 2, pp. 106-114. en_ZA
dc.identifier.issn 1024-5332 (print)
dc.identifier.issn 1607-8454 (online)
dc.identifier.other 10.1179/1607845412Y.0000000039
dc.identifier.uri http://hdl.handle.net/2263/58054
dc.language.iso en en_ZA
dc.publisher Maney Publishing en_ZA
dc.rights © W. S. Maney & Son Ltd 2013 en_ZA
dc.subject Flow cytometry en_ZA
dc.subject Platelets en_ZA
dc.subject Whole blood en_ZA
dc.subject Finger-prick en_ZA
dc.subject Storage en_ZA
dc.title Flow cytometric comparison of platelets from a whole blood and finger-prick sample : impact of 24 hours storage en_ZA
dc.type Postprint Article en_ZA


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